Sequences from confirmation amplicon


Confirmation sequences for line 103E02 and hit At5g67580 are shown.
Line ID 103E02
Locus At5g67580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26956665
Primers used for PCR PC41 - 8474, show primer details
   
Sequence Name 03-K103785-0022-103-E02C-PC41
GenBank Accession LN487457 [GenBank]
Sequencing primer gene specific primer, PC41: 5'-TCATCATCAAGTCTCTCTCTCGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K103785-0022-103-E02C-PC41
TCCTTCCTCCTTTCTGCTTCCTTCTCTGAATTTTCCATTTTTGTTTTTAATTTTCTACGA
TCGCCCCATTTGAATTTGGTTGATAATTTAGCTATCGCTTTAGATTGATGGCTGTATTTC
CAAATTCCTGTTGATTTGCGACGTTGTTAATGGGCTTTGATCCTCTTTTTTTGGTTCGAG
ATTGCTCGAATCACTCATTTTTTTGTTGAATTGAGCTTTGGGGCGTATCTAATTTCTTTG
GATTTGTGTAGTTACATTTTTATGTTGTGGCTTTGTGTATCTGCTGGATTTAGATGACCA
AAATTTTGTATTGGATAAGAAATGTTCAACAATGGCTTTGATGTGGTTAAGTTTCGTACG
ATTTAACAGATTCTAATGTTAGCGTTCACATCTATTGGCAGAGAAAAGAAGAAATTCAAA
TATTGATAACTGTGGATGGGTGCACCAAAGCAGAAGTGGACACCGGAAGAAGAAGCAGCT
CTTAAAGCTGGAGTTCTTAAGCATGGGACTGGGAAGTGGCGTACCATACTCTCGGATACT
GAGTTTAGTTTAATCCTTAAGTCTCGCTCTAATGTTGATCTTAAGGTAATACAATATAAT
ATGCTTTTTGGAGGTTGTGATCGCGTGTTTCGGACTAATCTTGATAGTTCAAGGCATAAC
AGGACAAATGGAGAAATATAAGCGTGACAGCTTTATGGGGTTCAAGGAAGAAGGCTAAAC
TTGCGCTTAAGAGGACTCCACCAGGTACNTAACAGGATGATAACAACACAGCTCTTACCA
TTGTGGCTTTGACTAATGATGATGAACGTGCAAAACAACTTCCCCCGGAGGTTCTGGTGG
TGGTC
   
Sequence Name 19-K103786-0022-103-E02C-8409
GenBank Accession LN494730 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K103786-0022-103-E02C-8409
CTTTACATTAATCGTTATGTGGTTCTTGACCACCACCAGAACCTCCGGGGGAAGTTGGTT
TTGCACGTTCATCATCATTAGTCAAAGCCACAATGGTAAGAGCTGTGTTGTTATCATCCT
GTTTAGTACCTGGTGGAGTCCTCTTAAGCGCAAGTTTAGCCTTCTTCCTTGAACCCCATA
AAGCTGTCACGCTTATATTTCTCCATTTGTCCTGTTATGCCTTGAACTATCAAGATTAGT
CCGAAACACGCGATCACAACCTCCAAAAAGCATATTATATTGTATTACCTTAAGATCAAC
ATTAGAGCGAGACTTAAGGATTAAACTAAACTCAGTATCCGAGAGTATGGTACGCCACTT
CCCAGTCCCATGCTTAAGAACTCCAGCTTTAAGAGCTGCTTCTTCTTCCGGTGTCCACTT
CTGCTTTGGTGCACCCATCCACAGTTATCAATATTTGAATTTCTTCTTTTCTCTGCCAAT
AGATGTGAACGCTAACATTAGAATCTGTTAAATCGTACGAAACTTAACCACATCAAAGCC
ATTGTTGAACATTTCTTATCCAATACAAAATTTTGGTCATCTAAATCCAGCAGATACACA
AAGCCACAACATAAAAATGTAACTACACAAATCCAAAGAAATTAGATACGCCCCAAAGCT
TCAATTCAACAAAAAAATGAGTGATTCGAGCAATCTCGAACCAAAAAAAGAGGATCAAAA
CCCATTAACAACGTCGCAAATTAACAGGAATTTGGAAATACAGC

Last Updated on 06.12.2018 16:11