Sequences from confirmation amplicon


Confirmation sequences for line 104A06 and hit At1g23290 are shown.
Line ID 104A06
Locus At1g23290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8263182
Primers used for PCR CB20 - 8474, show primer details
   
Sequence Name 10-K104526-0022-104-A06C-CB20
GenBank Accession LN490641 [GenBank]
Sequencing primer gene specific primer, CB20: 5'-GCGAGACTTTAGGGTTTATGAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K104526-0022-104-A06C-CB20
CAGGAGGCGCCGATCACTGTCGTCGAAAACTCAAAAGTGAAGTGGTTAACTAAACCTGAT
ATTAGGGATTCATATAATGGGCCTCATAGCCCAATTTAATAACATAAATAACCTGTGGTA
TAATTGGGCCAAATTATTAAAAAGCCCAAAAGACAATACACTATCTCTGTTTCTCCGAAA
ACCCTAGCTGCTAAATCCTCTCTATATAAAAACCCTTTAAGATCATTTCTGGTTCAAAAC
CCTAGATCTGATACTAAAATGGCGACGGCGTTGAAGAAGAACAGAAAGAAGAGAGGTCAC
GTCAGCGCCGGACATGGACGTATCGGTAAGCACCGTAAGCATCCCGGAGGTCGTGGAAAC
GCAGGAGGTATGCATCACCACAGGATCCTCTTCGACAAGTACCATCCAGGTTACTTCGGG
AAAGTTGGTATGAGTATATATTCAATATGGCTTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTA
   
Sequence Name 26-K104527-0022-104-A06C-8409
GenBank Accession LN497911 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K104527-0022-104-A06C-8409
CTTTACAATTGAAGCCATATTGAATATATACTCATACCAACTTTCCCGAAGTAACCTGGA
TGGTACTTGTCGAAGAGGATCCTGTGGTGATGCATACCTCCTGCGTTTCCACGACCTCCG
GGATGCTTACGGTGCTTACCGATACGTCCATGTCCGGCGCTGACGTGACCTCTCTTCTTT
CTGTTCTTCTTCAACGCCGTCGCCATTTTAGTATCAGATCTAGGGTTTTGAACCAGAAAT
GATCTTAAAGGGTTTTTATATAGAGAGGATTTAGCAGCTAGGGTTTTCGGAGAAACAGAG
ATAGTGTATTGTCTTTTGGGCTTTTTAATAATTTGGCCCAATTATACCACAGGTTATTTA
TGTTATTAAATTGGGCTATGAGGCCCATTATATGAATCCCTAATATCAGGTTTAGTTAAC
CACTTCACTTTTGAGTTTTTCGACGACAGTTGATCGGCGCCGTCCTTGACAGCTTCTCCT
TCTTCTTTTTCTTCACTCATAAACCCTAAAGTCTCG

Last Updated on 06.12.2018 16:11