Sequences from confirmation amplicon


Confirmation sequences for line 104G02 and hit At2g33100 are shown.
Line ID 104G02
Locus At2g33100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14040098
Primers used for PCR BL24 - 3144, show primer details
   
Sequence Name 02-K104808-0022-104-G02C-BL24
GenBank Accession LN487022 [GenBank]
Sequencing primer gene specific primer, BL24: 5'-AAACAAACAATCACCTGTAGGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K104808-0022-104-G02C-BL24
CTTTCCTCGTGAGAGTTTCCATGGCTTGTCTAGAAATCGGACTTGCTAACGCCACCATCA
TAGGTATCATCCTCTTCTGACCAAAACGCGTTTCCAATACCGTATTTTCCTTTAGATTCA
AACAACCACCTGTTATGATCAAAATCTCCCGTCTGGCTCCTCAACAACATTGATTTGTTA
TTTGATTTCATGACAGACAATCTCCTCTCCAGCTTATTCCCCATCTTGGGCCCGTCTCCT
CCTCCTCCACCTCGGTTAGCATCACCTTTAGACTCCGAGGTGGAGCCAGAGGAGCCAGCA
GGTTGGTTGTCAGGTGTTGGAGGCATGAGGACGGTGTAGTTGATGTAATCATTTTGGCCG
GAGTAATCACCAGAGACGTCCATGTCATCGTCACGGGAGAGGCTAACAATGCGACCACTG
GACGTCCGGCGACCAAACTTGACAGCTTGTGGAGGCCGGCTTAAGGAAGAAGATTGTGAA
TTTAAGGTCTTCTTGGGTGGACTTGAAGCCATTACAATTATGGACTGATAGTTTAAACCG
AAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTT
CCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACATCA
GTGGAGATA
   
Sequence Name 18-K104809-0022-104-G02C-3144
GenBank Accession LN494294 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K104809-0022-104-G02C-3144
TCCACATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTCCATAATT
GTAATGGCTTCAAGTCCACCCAAGAAGACCTTAAATTCACAATCTTCTTCCTTAAGCCGG
CCTCCACAAGCTGTCAAGTTTGGTCGCCGGACGTCCAGTGGTCGCATTGTTAGCCTCTCC
CGTGACGATGACATGGACGTCTCTGGTGATTACTCCGGCCAAAATGATTACATCAACTAC
ACCGTCCTCATGCCTCCAACACCTGACAACCAACCTGCTGGCTCCTCTGGCTCCACCTCG
GAGTCTAAAGGTGATGCTAACCGAGGTGGAGGAGGAGGAGACGGGCCCAAGATGGGGAAT
AAGCTGGAGAGGAGATTGTCTGTCATGAAATCAAATAACAAATCAATGTTGTTGAGGAGC
CAGACGGGAGATTTTGATCATAACAGGTGGTTGTTTGAATCTAAAGGAAAATACGGTATT
GGAAACGCGTTTTGGTCAGAAGAGGATGATACCTATGATGGTGGCGTTAGCAAGTCCGAT
TTCTTAGACAAGCCATGGAAACCTCTCACGAGGAAAGTTCAGATCCCGGCCAAAATTCTT
AGTCCCTAC
Primers used for PCR BL25 - 8474, show primer details
   
Sequence Name 03-K104808-0022-104-G02C-BL25
GenBank Accession LN487478 [GenBank]
Sequencing primer gene specific primer, BL25: 5'-GCTAAACCCTTGGTTTCTAGCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K104808-0022-104-G02C-BL25
TTTTATGTGTATCTTTGTTACATTTTCGATGATTTGATCTATCTAGTTTTCTCACAATGT
TATACACAATCTTTTAACCATTGATAAGCAACCTTCATTGAGATAGAGAGAGGCAAAGCA
CAAAGAAAGGAGATTTCAGAACTCAAAAGATTTCAGTTTTAAGTTTTCCTTATAATAGAA
AAGGATCTATTAACCGTTGAAATTCTAAAGCTTTTACATTTCCTTCCAAACTCATTCCTC
TGTTTTCTTATTTTCCTCTTTTCCTAAATCAGGTTTGTCAAAAAAAAATAAAAAAAAATG
GGTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCA
   
Sequence Name 19-K104809-0022-104-G02C-8409
GenBank Accession LN494750 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K104809-0022-104-G02C-8409
TCGGAATGAGCCATTTACATTGAACCCATTTTTTTTTATTTTTTTTTGACAAACCTGATT
TAGGAAAAGAGGAAAATAAGAAAACAGAGGAATGAGTTTGGAAGGAAATGTAAAAGCTTT
AGAATTTCAACGGTTAATAGATCCTTTTCTATTATAAGGAAAACTTAAAACTGAAATCTT
TTGAGTTCTGAAATCTCCTTTCTTTGTGCTTTGCCTCTCTCTATCTCAATGAAGGTTGCT
TATCAATGGTTAAAAGATTGTGTATAACATTGTGAGAAAACTAGATAGATCAAATCATCG
AAAATGATAACAAAGATACACATAAAAACACTCTCTCTAATACAATATCAGACTCTAAAG
CTAGAAACCAGGGGTTTA

Last Updated on 06.12.2018 16:11