Sequences from confirmation amplicon


Confirmation sequences for line 105E08 and hit At3g47800 are shown.
Line ID 105E08
Locus At3g47800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17635581
Primers used for PCR KT20 - 8474, show primer details
   
Sequence Name 01-K026965-022-105-E08C-8409
GenBank Accession LN486430 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K026965-022-105-E08C-8409
TGATCCATGTAGATTTCCCGGACATGAAGCCATTTACAAATGGGATCCATTGTGGGAAGA
GTGGCTAATAGAATAGGAGGTGCTAAATTCAAGTTAAATGGTCATCTCTACAAAACCGAT
CCCAACGAAGGCCGTAACACTCTCCATGGTACTCAAAACCGATCGCATGAGATAAATCTT
CAATTGAATTTCATTTCTATAAATTGTGTTATTGATGATGGTTTTTGTTGTTTAATAATA
ATCAGGTGGTTCAAAGGGATTTAGTGATGTGATTTGGTCAGTCCAAAAGTATGTTCCCAC
TAGTCACATTACTTTCACATACGATAGCTTCGACGGTGAAGAAGGTAAATCT
   
Sequence Name 02-K026965-022-105-E08C-KT20
GenBank Accession LN486888 [GenBank]
Sequencing primer gene specific primer, KT20: 5'-CTAGATTTACCTTCTTCACCGTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K026965-022-105-E08C-KT20
ATCGTATGTGAAAGTAATGTGACTAGTGGGAACATACTTTTGGACTGACCAAATCACATC
ACTAAATCCCTTTGAACCACCTGATTATTATTAAACAACAAAAACCATCATCAATAACAC
AATTTATAGAAATGAAATTCAATTGAAGATTTATCTCATGCGATCGGTTTTGAGTACCAT
GGAGAGTGTTACGGCCTTCGTTGGGATCGGTTTTGTAGAGATGACCATTTAACTTGAATT
TAGCACCTCCTATTCTATTAGCCACTCTTCCCACAATGGATCCCATTTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11