Sequences from confirmation amplicon


Confirmation sequences for line 106D02 and hit At3g60530 are shown.
Line ID 106D02
Locus At3g60530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22373694
Primers used for PCR 09ns - 3144, show primer details
   
Sequence Name 64-K114716-0022-106-D02C-09ns
GenBank Accession LN511256 [GenBank]
Sequencing primer gene specific primer, 09ns: 5'-CACATTCTTTTCACCTCATTCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K114716-0022-106-D02C-09ns
GCAAATGTCCGTAACATAAAAATTGAAGTATTATATGCTTTTACTGTTTAGAGTGTCTGT
TTTGAAAGAAATGTTTTCAAAATGCTTGTTTAGAATATAAGCTCGATCTATAATATATGC
CGTTTTTAAAAAAAGACTAGTATATGCATTATGTAATTCATGGTTAATTAATCCTCTGGT
AAGTAAATTAAATAGCACATGCATGTTCAAAGAAAAATGTAGACAAATATATTAAAGTGG
ATAAATAAATAGAAAAAAAGGGAGTGGTCAGGTCAGTCTCGGTGCATGCTAACTCTATGC
ACGTTCTCATCTTCGTCTCTTTCCTCACTTACACTAATTCTCTCTCTCTATATTAAACCT
CTGAACCAGTGACCACATACATACTCAGTAAGCGAGTCCGAGTCAACAATGGACGTCTAC
GGCATGTCTTCACCGGACTTGCTTCGTATCGACGACCTTCTCGATTTCTCCAACGACGAA
ATCTTCTCTTCCTCTTCCACCGTCACTTCCTCCGCCGCTTCCTCCGCCGCTTCTTCCGAA
AACCCTTTCAGCTTTCCTTCTTCCACCTACACTTCTCCTACTCTCCTCACCGACTTCACT
CACGATCTCTGCGTTCCCGTACGTTTTCAAATTTCAATTTACCCTAATTTTGATTTTGAT
TTTGAATTTCTAAGTTCTGATTTTGAGTTTTGCAGAGTGACGACNCANCTCATCTCGAAG
GGTTATCGCGATTCGTTGACGATNCATTCTCCGATAGTTTAAACCGAAGGCGGGAAACGA
CAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCNTATATNGAGGA
AGGGTCTTGCGAAGGAAAGTGGGAATGTGCGTCATCCC
   
Sequence Name 16-K114715-0022-106-D02C-3144
GenBank Accession LN493534 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K114715-0022-106-D02C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCGGAGAATGAAT
CGTCAACGAATCGCGATAACCATTCGAGATGAGCTGCGTCGTCACTCTGCAAAACTCAAA
ATCAGAACTTAGAAATTCAAAATCAAAATCAAAATTAGGGTAAATTGAAATTTGAAAACG
TACGGGAACGCAGAGATCGTGAGTGAAGTCGGTGAGGAGAGTAGGAGAAGTGTAGGTGGA
AGAAGGAAAGCTGAAAGGGTTTTCGGAAGAAGCGGCGGAGGAAGCGGCGGAGGAAGTGAC
GGTGGAAGAGGAAGAGAAGATTTCGTCGTTGGAGAAATCGAGAAGGTCGTCGATACGAAG
CAAGTCCGGTGAAGACATGCCGTAGACGTCCATTGTTGACTCGGACTCGCTTACTGAGTA
TGTATGTGGTCACTGGTTCAGAGGTTTAATATAGAGAGAGAGAATTAGTGTAAGTGAGGA
AAGAGACGAAGATGAGAACGTGCATAGAGTTAGCATGCACCGAGACTGACCTGACCACTC
CCTTTTTTTCTATTTATTTATCCACTTTAATATATTTGTCTACATTTTTCTTTGAACATG
CATGTGCTATTTAATTTACTTACCAGAGGATTAATTAACCATGAATTACATAATGCATAT
ACCAGTCTTTTTTTAAAAACGCATATNTNATAGATCGAGCTTATATTCTAAACAAGCATT
TTGAAAACATTTCTNNTCAAACAGACACTCTAAACAGTAAAAGCATATNANACTTCAATT
TTTATTGTTACGGACATTTTTGCCCTTAACTATAAACTGG

Last Updated on 06.12.2018 16:11