Sequences from confirmation amplicon


Confirmation sequences for line 106H02 and hit At5g56530 are shown.
Line ID 106H02
Locus At5g56530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22890309
Primers used for PCR 28ym - 8474, show primer details
   
Sequence Name 15-K120546-0022-106-H02C-28ym
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 28ym: 5'-CACACTCATTGTGCCTATGTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K120546-0022-106-H02C-28ym
CTCCAGAATCGCTGTTTGGTGAGAGCAAAGTGTCTGAGAAACCAAAAGAGAGTGTCAATC
CTATTACACAGTTATGGCACCAGAACGGAGTTTGTTCTGAAGGGACAATACCAGTGAGGA
GGACAAAGAAAGAGGATGTCTTGAGGGCGAGTTCTGTTAAACGGTATGGTAAGAAGAAGC
ACCTTTCTGTTCCTCTGCCTCGATCTGCTGATCCTGATCTCATTAACCAAAGTGGTCATC
AGGTAGACTTTAGTGATCTTTATCTATATACCTATGTATTATGTGAATTCTTTATCTAAG
GGTTCTGTTTTGGGTTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATG
   
Sequence Name 05-K120545-0022-106-H02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K120545-0022-106-H02C-8409
CTTTACATTGAATATAACCCAAACAGAACCCTTAGATAAAGAATTCACATAATACATAGG
TATATAGATAAAGATCACTAAAGTCTACCTGATGACCACTTTGGTTAATGAGATCAGGAT
CAGCAGATCGAGGCAGAGGAACAGAAAGGTGCTTCTTCTTACCATACCGTTTAACAGAAC
TCGCCCTCAAGACATCCTCTTTCTTTGTCCTCCTCACTGGTATTGTCCCTTCAGAACAAA
CTCCGTTCTGGTGCCATAACTGTGTAATAGGATTGACACTCTCTTTTGGTTTCTCAGACA
CTTTGCTCTCACCAAACAGCGATTCTGGAGTGTAACTAGGTCCCATCTGTTTAAAACCCC
ATAACATGTTAAGACATAGGCACAATGAGTGTGATGGT

Last Updated on 06.12.2018 16:11