Sequences from confirmation amplicon


Confirmation sequences for line 106H04 and hit At5g04070 are shown.
Line ID 106H04
Locus At5g04070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1102886
Primers used for PCR 16ra - 8474, show primer details
   
Sequence Name 75-K115895-0022-106-H04C-16ra
GenBank Accession LN513293 [GenBank]
Sequencing primer gene specific primer, 16ra: 5'-CTCCAGTAACAGAATCCATGTCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K115895-0022-106-H04C-16ra
CATATACCAGAAAGACATAACTATCTTATTCTCATTTAGGACGCAAAATCATTCAATCAG
ATTGATTATGAGAAACTATTCACCAGAACGATGTGTAAAAGATGTAACATTCACTACTCT
TGAAGGCACAGGGCTGTTTCTCAGGAGCGGTAAGAGAAGTTTGGTCAAAGAGAAAGCGCC
GACATAATTTGTAGCAATCATCCTGAAAACGATGGAGACTGAAAGTGTAATCAATAGAGT
TCAAAATTCTGAAACCATAGTGAAAAGTGAAAACCTTACCTGTCAAAGCCTTCAACAGTT
GGACGACTAGATGTTGCCAGTATTCCAGCATTGTTCACCAAAAGCTGGACTGAAGAATGC
AAATCTGACTCAAAAAGCCATTGCTCTAGAGAGCTTCTGAACTTTAAAACTAATTGAAAA
GACGACATGTCAACTTCAAAAGCTTTGAGTTTGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 45-K115894-0022-106-H04C-8409
GenBank Accession LN505644 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K115894-0022-106-H04C-8409
CAACTCAAGCTTTTGAGTTGACATGTCGTCTTTTCATTAGTTTTAAAGTTCAGAAGCTCT
CTAGAGCAATGGCTTTTTGAGTCAGATTTGCATTCTTCAGTCCAGCTTTTGGTGAACAAT
GCTGGAATACTGGCAACATCTAGTCGTCCAACTGTTGAAGGCTTTGACAGGTAAGGTTTT
CACTTTTCACTATGGTTTCAGAATTTTGAACTCTATTGATTACACTTTCAGTCTCCATCG
TTTTCAGGATGATTGCTACAAATTATGTCGGCGCTTTCTCTTTGACCAAACTTCTCTTAC
CGCTCCTGAGAAACAGCCCTGTGCCTTCAAGAGTAGTGAATGTTACATCTTTTACACATC
GTTCTGGTGAATAGTTTCTCATAATCAATCTGATTGAATGATTTTGCGTCCTAAATGAGA
ATAAGATAGTTATGTCTTTTCTTGGTATATGTTTTTCTATGTAGCTTTTACTGGGAGGTT
TGACATGGATTCGNTAACTGGA

Last Updated on 06.12.2018 16:11