Sequences from confirmation amplicon


Confirmation sequences for line 107A07 and hit At5g04740 are shown.
Line ID 107A07
Locus At5g04740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1369208
Primers used for PCR 03kq - 8474, show primer details
   
Sequence Name 54-K113756-0022-107-A07C-03kq
GenBank Accession LN508543 [GenBank]
Sequencing primer gene specific primer, 03kq: 5'-TGTTTTAGAGCAAACAAAAGCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K113756-0022-107-A07C-03kq
CCTTCTCGGAAGTAACGCAGACAGTTCACCAAAACCTGCAGGAGATAGTTGATTCTTTTT
ACCTCTCTCTCTTCTTTTAAAGGTGCAAAATACAAGACTAACAGAAACAAAAACTTACCT
GTGACAAAGATCTGTTTAGAGCTTGCCCTTGGTAGCTTACGTGAAACTTGTCTTTCGCAA
CCAGTCCCTGAGAGAGACAATGGAGACGTACTATTATAATGCATAGCATAGAAATGGAAT
CACAGTGTATGTACTTATGTAGGTTTTGACTACCTCTGTATCTATCTCTGCGGATTCCAC
GTCTATGTTGACATCAGCCATCACTTTGATCATCTCTACGACAAGACCTGGCCTGTCCGC
TGTCTCTATGACAAGCAGGCTAGAGAAAACAGGAAACAGGCGAGAATTATGTAAACTATT
TCAACAGGGCTATTCTTTATATATGATTTTGTGGTGTCATTAATAATGCTAATAAATATG
GTGAATACCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 16-K113755-0022-107-A07C-8409
GenBank Accession LN493508 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K113755-0022-107-A07C-8409
CATTATTAATGACCCACAAAATCATATATAAAGAATAGCCCTGTTGCCTTATTTACATAA
TTCTCGCCTGTTTCCTGTTTTCTCTAGCCTGCTTGTCATAGAGACAGCGGACAGGCCAGG
TCTTGTCGTAGAGATGATCAAAGTGATGGCTGATGTCAACATAGACGTGGAATCCGCAGA
GATAGATACAGAGGTAGTCAAAACCTACATAAGTACATACACTGTGATTCCATTTCTATG
CTATGCATTATAATAGTACGTCTCCATTGTCTCTCTCAGGGACTGGTTGCGAAAGACAAG
TTTCACGTAAGCTACCAAGGGCAAGCTCTAAACAGATCTTTGTCACAGGTAAGTTTTTGT
TTCTGTTAGTCTTGTATTTTGCACCTTTAAAAGAAGAGAGAGAGGTAAAAAGAATCAACT
ATCTCCTGCAGGTTTTGGTGAACTGTCTGCGTTACTTCCTGAGAAGGCCTGAAACTGACA
TTGACAGCTACTAAATGAGCTTTTGTTTGCTCTAAAACAAAAGCTCATTTAGTAGCTGTC
AATGTCAGTTTCAGGCCTTCTCAGGAAGTAACGCAGACAGTTCACCAAAACCTGCAGGAG
ATAGTTGATTCTTTTTACCTCTCTCACGTCTTTTAAAGGTGCAACGTACAAGATTAACAG
AACC

Last Updated on 06.12.2018 16:11