Sequences from confirmation amplicon


Confirmation sequences for line 107A12 and hit At5g07130 are shown.
Line ID 107A12
Locus At5g07130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2211942
Primers used for PCR 07pm - 8474, show primer details
   
Sequence Name 38-K115064-0022-107-A12C-07pm
GenBank Accession LN503089 [GenBank]
Sequencing primer gene specific primer, 07pm: 5'-TTTCTAGTGGGGTCAGGAGAGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K115064-0022-107-A12C-07pm
GACTCTTCTTTGGTGTCGCGAATCAAAACTCACAGTGGTTGCGGCGGATGCCTCCTACAC
CAAACCATTCTCGACCAATGTCATAATGTTAGGTCCTGGTCAGACAACTGATGTGCTTCT
AACTGCAGACCAACCGCCAGCACACTACTACATGGCAGCACATGCCTACAACAGTGCCAA
TGCTGCCTTTGACAACACCACAACCACCGCAATCCTCAAGTACAAAGACGCCTCCTGTGT
TACTCTTCAAGCTAAATCACAGGCACGGGCAATACCCGCACAACTACCAGGATTCAACGA
CACAGCAACAGCAGCAGCCTTCACAGCGCAGATGAAGAGTCCTTCGAAAGTAAAAGTGCC
CCTGGAGATTGATGAGAACTTGTTTTTCACAGTGGGATTGGGACTATTCAACTGCCCAAC
CCCCAACACACAAAGAAAATGAGTATAAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGC
   
Sequence Name 06-K115063-0022-107-A12C-8409
GenBank Accession LN488933 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K115063-0022-107-A12C-8409
ATTTTATACTCATTTTCTTTGTGTGTTGGGGGTTGGGCAGTTGAATAGTCCCAATCCCAC
TGTGAAAAACAAGTTCTCATCAATCTCCAGGGGCACTTTTACTTTCGAAGGACTCTTCAT
CTGCGCTGTGAAGGCTGCTGCTGTTGCTGTGTCGTTGAATCCTGGTAGTTGTGCGGGTAT
TGCCCGTGCCTGTGATTTAGCTTGAAGAGTAACACAGGAGGCGTCTTTGTACTTGAGGAT
TGCGGTGGTTGTGGTGTTGTCAAAGGCAGCATTGGCACTGTTGTAGGCATGTGCTGCCAT
GTAGTAGTGTGCTGGCGGTTGGTCTGCAGTTAGAAGCACATCAGTTGTCTGACCAGGACC
TAACATTATGACATTGGTCGAGAATGGTTTGGTGTAGGAGGCATCCGCCGCAACCACTGT
GAGTTTGTGATTCGCGACACCAAAGAAGAGTTCTTGGTTCAAAGCAGAGTTGATGACCCT
GAGGAGGACTATCTCTCCTGACCCCACTAGAA

Last Updated on 06.12.2018 16:11