Sequences from confirmation amplicon


Confirmation sequences for line 107E04 and hit At4g26540 are shown.
Line ID 107E04
Locus At4g26540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13396288
Primers used for PCR 06vd - 3144, show primer details
   
Sequence Name 76-K117751-0022-107-E04C-06vd
GenBank Accession LN513457 [GenBank]
Sequencing primer gene specific primer, 06vd: 5'-GAGTTTTTGGTAAAGCGTCACTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K117751-0022-107-E04C-06vd
GTTTTCCGGCGGCTCTTGCCCGGACTAGAGTGTAAACCGCCATGAGAACAAGTACGGCAG
TGACGACGACGAGAATTAAAATAGTCAATCTAACGACGGAGCTGTTCCTGGTCGTCGGGT
CGGGTCGGGTCGAGATTGCGTTCGAGATGTAAAGCCCTCTGTTTGAGGCGAGATCAGAAA
GTGGAAGTCGCCGGAAAAAGGGCGTGTTGGGTAAATCGCCGGAGAAATCGTTGTAGGAGA
TGTTGAGAGAGACGAGGTTTTGCAAATCCGTTAAAACGTTTAAGTTTCCGGTGAGTTGGT
TGTGAGATACGTCGAGCACTCCAAGGTTTTTCAAGTCGGAGAATCTAGACGGGATTTCTC
CGACGAACCTATTGCAGCTGAGATTAAGAGATATCGCCAGAGACGGGATTTGACCCAATT
CGTCAGGGATTTCACCAGAAAAGTCATTTTCGCCAAACACTGATAGTTTAAACCGAAGGC
GGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTA
TATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGA
GATA
   
Sequence Name 51-K117750-0022-107-E04C-35St
GenBank Accession LN507625 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K117750-0022-107-E04C-35St
CTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGGCGAAAATGAC
TTTTCTGGTGAAATCCCTGACGAATTGGGTCAAATCCCGTCTCTGGCGATATCTCTTAAT
CTCAGCTGCAATAGGTTCGTCGGAGAAATCCCGTCTAGATTCTCCGACTTGAAAAACCTT
GGAGTGCTCGACGTATCTCACAACCAACTCACCGGAAACTTAAACGTTTTAACGGATTTG
CAAAACCTCGTCTCTCTCAACATCTCCTACAACGATTTCTCCGGCGATTTACCCAACACG
CCCTTTTTCCGGCGACTTCCACTTTCTGATCTCGCCTCAAACAGAGGGCTTTACATCTCG
AACGCAATCTCGACCCGACCCGACCCGACGACCAGGAACAGCTCCGTCGTTAGATTGACT
ATTTTAATTCTCGTCGTCGTCACTGCCGTACTTGTTCTCATGGCGGTTTACACTCTAGTC
CGGGCAAGAGCCGCCGGAAAACAACTCCTCGGAGAAGAAATAGATTCGTGGGAAGTGACG
CT

Last Updated on 06.12.2018 16:11