Sequences from confirmation amplicon


Confirmation sequences for line 108C10 and hit At2g28160 are shown.
Line ID 108C10
Locus At2g28160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12004935
Primers used for PCR H29a - 8474, show primer details
   
Sequence Name 37-K014707-022-108-C10C-8409
GenBank Accession LN502423 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K014707-022-108-C10C-8409
ATTTCCCGGACATGAATTGCCTGACTTAGACTCCAACGTTGCTGAATCATTCCGTAGCTT
CGACGGTGATAGTGTTAGAGCCGGTGGTGAAGAAGATGAAGAAGATTACAACGACGGTGA
TGATTCTTCAGCCACTACTACGAATAATGATGGGACCCGTAAGACGAAGACTGATCGGTC
TAGGACTTTGATCTCTGAGAGAAGAAGGAGAGGGCGTATGAAGGATAAGCTTTATGCATT
GAGATCTCTTGTTCCCAATATTACTAAGGTAAGTCACAACATTTTCAAGATTTGGTAATG
ACTAATGAAACTGTAAATATTGATGAAGTATGTGTGTGTTGGCATTTAGATGGATAAAGC
ATCC
   
Sequence Name 38-K014707-022-108-C10C-H29a
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, H29a: 5'-GAGGATTGATACCGCGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K014707-022-108-C10C-H29a
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACG

Last Updated on 06.12.2018 16:11