Sequences from confirmation amplicon


Confirmation sequences for line 108H02 and hit At4g14390 are shown.
Line ID 108H02
Locus At4g14390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8290594
Primers used for PCR IH58 - 8474, show primer details
   
Sequence Name 78-K100854-0022-108-H02C-8409
GenBank Accession LN513648 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K100854-0022-108-H02C-8409
GCCATTTACATTGAATATATCCTGTACATGTCCAGGATATACCTATGATTTTCAAAATTG
CTTTCACAAGAGATACATCTCCAGTATTTATTGCCACAAACAAGGAAGATACTCCATACT
TATTTCCAAGAAAAGGAGCATCCTGGTTTGCGTTCACCAGACATACAGCCATCTCGAAAT
AGCGCCCTTCAATCGCGTAGTACAGAGCAGTATTTCCATCTTTGTCCTTGAGAACATATG
GGTTCATTCTCTCACTCTCTTCATTACACAGTCTAGCTGAAGAAAATGTTACCAATGCGA
CAAAAGCCTCAACAATAGTGGTATGACCGCTATGAGCCGCCACATGTAGCGGTGTCTGAC
CGCTTGAGTTTTGCTCCAAGAGAAGACGCGGGCATTCACAGACAATCTCCTTCACTAGTT
CTAGATGACCCCATGTAACAGCAAGATGAAGAATTGAATCTCCTGTATTGCTCTTGATAC
GTGCCACTGAAATTCCACGGCTTCTCAACTTCTCCAGACATTCTTTGTTCCCATCACTTA
TTTTACTAAAAACCTCTGGAATCATCCGGACATCTTCACCACGGATAGCGTAAAGATCCG
ACAATCTAAGGTTAGTAAGG
   
Sequence Name 78-K100860-0022-108-H02C-IH58
GenBank Accession LN513649 [GenBank]
Sequencing primer gene specific primer, IH58: 5'-TGTGCCAGAGTTCCTTACTAACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K100860-0022-108-H02C-IH58
TGTCCGGATGATTCCGAGGTTTTTAGTAAAATAAGTGATGGGAACAAAGAATGTCTGGAG
AAGTTGAGAAGCCGTGGAATTTCAGTGGCACGTATCAAGAGCAATACAGGAGATTCAATT
CTTCATCTTGCTGTTACATGGGGTCATCTAGAACTAGTGAAGGAGATTGTCTGTGAATGC
CCGCGTCTTCTCTTGGAGCAAAACTCAAGCGGTCAGACACCGCTACATGTGGCGGCTCAT
AGCGGTCATACCACTATTGTTGAGGCTTTTGTCGCATTGGTAACATTTTCTTCAGCTAGA
CTGTGTAATGAAGAGAGTGAGAGAATGAACCCATATGTTCTCAAGGACAAAGATGGAAAT
ACTGCTCTGTACTACGCGATTGAAGGGCGCTATTTCGAGATGGCTGTATGTCTGGTGAAC
GCAAACCAGGATGCTCCTTTTCTTGGAAATAAGTATGGAGTATCTTCCTTGTTTGTGGCA
ATAAATACTGGAGATGTATCTCTTGTGAAAGCAATTTTGAAAATCATAGGTATATCCTGG
ACATGTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11