Sequences from confirmation amplicon


Confirmation sequences for line 109C05 and hit At2g33170 are shown.
Line ID 109C05
Locus At2g33170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14056867
Primers used for PCR KE11 - 3144, show primer details
   
Sequence Name 63-K107430-0022-109-C05C-KE11
GenBank Accession LN511004 [GenBank]
Sequencing primer gene specific primer, KE11: 5'-AACACTACTTCTCTCATCGTCGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K107430-0022-109-C05C-KE11
ACGGCATTTTCGTCACTGTGATCATATGATTGAGAATCACATCGTCCTCTACCTTTGTTA
GATAAGGATCAAGGATTTCAGATGTTAACGAATGATCTCTGATATGGTTTCTTGTCCACG
TGGCAAGATCTCCGCCTTGCTCTAGCGGCTGAACCGGAGCTTTCCCGGTTAACAATTCAA
GAAGAACAACTCCAAAGCTGTAGATATCGCATTTCTCTGTTACTTTCATTGTGTATGCAT
ACTCTGCAACAAAAAAAAAACAGTTAAAAAACGGTTAGCTTTAAGTTAGTGTAAACTCGA
AAGTTTAACGGTTTTTTACAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCT
GATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGAAAGGGTC
TTGCAAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAAATA
   
Sequence Name 47-K107429-0022-109-C05C-3144
GenBank Accession LN506213 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K107429-0022-109-C05C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGTA
AAAAACCGTTAAACTTTCGAGTTTACACTAACTTAAAGCTAACCGTTTTTTAACTGTTTT
TTTTTTGTTGCAAAGTATGCATACACAATGAAAGTAACAAAAAAATGCGATATCTACAGC
TTTGGAGTTGTTCTTCTTGAATTGTTAACCGGGAAAGCTCCGGTTCACCCGCTAGAGCAA
GGCGGAAATCTTGCCACGTGGACAAAAAACCATATCAAAAATCATTCGTTAACATCTGAA
ATCCTTGATCCTTATCTAACAAAGGTAGAGGACAATGTGATTCTCAATCATATGATCACA
GTGACAAAAATTGCCGTGCTCTGTACAAAATCTTCCCCGTCTGATCGACCGACAATAA
Primers used for PCR KE10 - 8474, show primer details
   
Sequence Name 64-K107430-0022-109-C05C-KE10
GenBank Accession LN511214 [GenBank]
Sequencing primer gene specific primer, KE10: 5'-GATAGCTACATTGTCGGTAGAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K107430-0022-109-C05C-KE10
GCCTTCAGGTAAACATAGCAGTTAAAAAACTCGAATCCAACAGAGAAGGCAACAACAACA
ACAGCAACAACACTGATAACAGTTTCCGCGCCGAGATTCTAACACTAGGCAAAATCCGTC
ACAGGAACATTGTCAGGCTATACAGTTTCTGTTACCACCAAGGATCAAACTCGAACCTCT
TGCTCTACGAATACATGTCTCGGGGAAGCCTTGGCGAGTTACTTCACGGCGGGAAATCAC
ATAGCATGGATTGGCCAACTCGGTTTGCTATCGCACTCGGTGCAGCGGAAGGTCTCGCTT
ACTTACATCATGATTGTAAACCAAGAATCATTCACCGTGACATCAAATCGAATAACATTC
TCATCGATGAGAATTTCGAAGCCCATGTTGGAGATTTCGGGTTAGCAAAAGTCATCGACA
TGCCACTATCAAAATCCGTCTCAGCCGTCAGCTAAAGAAGTCAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNT
TATT
   
Sequence Name 48-K107429-0022-109-C05C-8409
GenBank Accession LN506517 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K107429-0022-109-C05C-8409
GCCATTTACAATTGAATATATCCTGACTTCTTTAGCTGACGGCTGAGACGGATTTTGATA
GTGGCATGTCGATGACTTTTGCTAACCCGAAATCTCCAACATGGGCTTCGAAATTCTCAT
CGATGAGAATGTTATTCGATTTGATGTCACGGTGAATGATTCTTGGTTTACAATCATGAT
GTAAGTAAGCGAGACCTTCCGCTGCACCGAGTGCGATAGCAAACCGAGTTGGCCAATCCA
TGCTATGTGATTTCCCGCCGTGAAGTAACTCGCCAAGGCTTCCCCGAGACATGTATTCGT
AGAGCAAGAGGTTCGAGTTTGATCCTTGGTGGTAACAGAAACTGTATAGCCTGACAATGT
TCCTGTGACGGATTTTGCCTAGTGTTAGAATCTCGGCGCGGAAACTGTTATCAGTGTTGT
TGCTGTTGTTGTTGTTGCCTTCTCTGTTGGATTCGAGTTTTTTAACTGCTATTGTTTTAC
CTGAAGGCATAACTGCTTTGTAAACGGTCCCGCAAGCGCCTCTACCGACAATGTAGCTAT

Last Updated on 06.12.2018 16:11