Sequences from confirmation amplicon


Confirmation sequences for line 109D09 and hit At4g19260 are shown.
Line ID 109D09
Locus At4g19260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10535565
Primers used for PCR 72pm - 3144, show primer details
   
Sequence Name 89-K115121-0022-109-D09C-72pm
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 72pm: 5'-TCTAATGTGTTTGGTTTTCCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K115121-0022-109-D09C-72pm
CTAGGAGCTTTGCGTTGTTTGCCAATGAGCTTATCATAGACCCATACGTCAGTCGAGGGT
GTCATGTTTTGTCCACCGATTTCGTATGTTTCTGAACCAACAGTTTCGTGGACTATTTGT
AAGCAACGAGAATAGGGAGAGGGCATCGGTAGCAACCAATGTCCGGTACACTTGATGTCG
ATCGTCCAATGGGTTAGGGGCTGATGATCATAGGGTTTTATCCAGAGACCGAACCATCTA
CGATCAAAAGGATGGGTGGGAGATTGAAGGCAGACATAAACACATGGTTTTCGGGTTCCA
AGGTAGGAACGTTCCATGTCGAGCTCCTTGGAGAGGATGAGCAAACGGAAGGTCTTGGAG
ACAAGAGAGAGCGTTTGGTAGTAGGAAGTAGATATGCGAGCCAAGATATTCAAAACGATG
TCATCTGGTAGTGAAGAAAACAACGACGGAGATGGTTGGGGAGGCGGACAAGGAGTGTTT
GACTCGCGTGGTGTACGTTCCATATTAGACTTGAAGCTTAAGTATGCCATTAGTTTTTTT
ACAAAACAAACACTTGCTTTTTGTTTTCTAATCGAACACCGATCAAAATCTTATTGTATG
CATGTGAATATTTTATAGCCTAGATATATCCTTTTTTTTTTCACCAGCCTAAATAAACTC
TCCTTTTTTTTGGCTTTCCGGTTTTCCACCACCCAAAATATATCTCCAATATCCCCGGGG
GGGTCCCATATAAAACTTAAACCTAAATTATGCCATTATTTTTTTACAAAACAAACACTT
GCTTTTTGTTTTCTTACCGAACACCAATAAAAA
   
Sequence Name 41-K115120-0022-109-D09C-3144
GenBank Accession LN504187 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K115120-0022-109-D09C-3144
CTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAATGTTTGTTCATTCAAATAAATAATG
GAGTGTATTTTAGAATCAAATAAAAAAAAACTTTATCAAATGATTTATTTGGTAGATAAC
AACCAAATCTTGAGCTAAAAAGGGTGAAAAAATATTGAGAATTTTGTTAATAAGTAAATA
GTTATGGTTTTGACTGGGTATAGGAGATATATCTAGGCTGGTGAAAAAAAAAAGAAAAAA
AAGGATATTTCTGGGCTTTAAAATATTCACATGCATACAATCAAATTTTCATCGGTGTTC
GATAAGAAAACAAAAAGCAAGTGTTTGTTTTGTAAAAAACTAATGGCATACTTAAGCTTC
AATTCTAATATGGAACCCCCACGGGGATATAGGAAATATATCTAGGCTGGTGAAAAACAG
AAAAGCAAAAAAAAGGGAAATTTATTCTGGGCGGGGAAAAAAAAAAGGGATTTTTCGGGG
TAAAAAAATTTCCCCTGGCAAAAAAAAAAATTTGAACGGGGTTCCAATAAAAAAAAAAAA
ACAAGGTTTTTTTTTTAAAAAAAAATAAGGGGATAATTAAGCTTTCAGTTCAAAAAGGAA
AGTTCCCCCCCCGGGTTAAAACCTCCTTGGCCCCCCCCCCAAACATTCCCGGTCTTTTTT
TTCTCCACACCAAAAAAACTCCTTTTGAAATTTTGGGG

Last Updated on 06.12.2018 16:11