Sequences from confirmation amplicon


Confirmation sequences for line 109E03 and hit At1g73620 are shown.
Line ID 109E03
Locus At1g73620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27681381
Primers used for PCR AC98 - 8474, show primer details
   
Sequence Name 03-K104226-0022-109-E03C-AC98
GenBank Accession LN487469 [GenBank]
Sequencing primer gene specific primer, AC98: 5'-CATCGAGAGGTGAGATAAGTTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K104226-0022-109-E03C-AC98
AACCATATTCGTTAGACGTTACGTTCGTTGTCTACACAACAATGTAAAATAACTTTAAAA
AAAAAAAAGTAAGTAACTAAATGTACAAATCTTCTTTAGGACTGAGGGATTTAAATGGAT
ATATGATAGTTACTAGTTACGTTGTTAAAAAAAAGGGTATTAAATCACTAACAACGAAAA
ATTCATTTTATAGCTCCAAGTCAATACAATAATTTTAGTTTTCACCTAACAATATAAATA
GTTGATAATTCTCTACTTTCAACTTATTTCTGGTACAAACCGTATTATGTAAGGTAGGTA
AGCTTATTCTCAGGATAAGCTCATTTGATCTAATTTTAATATTTATATAAAAAACTTATA
AACCCTAATAAAAAAATATCTGACCAAAAAAAAAAAAAACCCCCAAAAAAAAAAAAAACG
GCCCCTTTTTTTTCCCCCTTAAAAAAAAAAATCCCCCAAACCCTGGCCCAAGGGAAAAGG
GGGATTTCCCCCCCCCACAA
   
Sequence Name 19-K104227-0022-109-E03C-8409
GenBank Accession LN494741 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K104227-0022-109-E03C-8409
GCCATTTCATTACATTGGAAGAGCAAAATAGCATTAGTTTCCAAAATGACATGATGTGAT
TAAGCCATTCTGATTTCAACTTTAGTGGAAGAGCAACTTCACATTTCTCATTGGTCATTG
CTTTTGGAGATTTTCTCTTTATAGAGAGAAAAAACAGTGTACTGTTTGTTTTTTATTAGG
GTTTTTTTTTTTTTTTGGGCAAAATTTTTTTTTTTGGGGTTTAAAATTTTTTTTAAAAAA
ATTTAAAATTTAAACAAAAGGACCAAACCAGGAAAAAAACCTTCCCCCCTTAAAAAAAAA
GGGTTTGTAAAAAAAAAATTTGAAAAGAAAAAATTTTCCACCTTTAAATTTGGTTGCCGA
AAAACAAAATTTTCCTTTTGGCCAAGACCCTAAAAAAGAATTTTTCCGTGGTAGGGGATT
AAAACCCTTTTTTTTAAACACCGAACCTGGAACCTACAAAATTTCATTCAAACCCCCCCC
CCCCAAAAAAAAATTGGACCTTTTGGTCCCTACTTTTTTTTTTTAAAAATTTTTTTAACT
TGGTTTTTAAAAAAAAAAAATTTAACCTTTTAAAAAAAATTTTTTTTGGGGAATTTTATA
AGGGAAAAACTTTTTCCCC

Last Updated on 06.12.2018 16:11