Sequences from confirmation amplicon


Confirmation sequences for line 110D06 and hit At1g73210 are shown.
Line ID 110D06
Locus At1g73210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27529564
Primers used for PCR CD50 - 8474, show primer details
   
Sequence Name 29-K104189-0022-110-D06C-CD50
GenBank Accession LN499264 [GenBank]
Sequencing primer gene specific primer, CD50: 5'-TGTGAGTTTTCAGATGAGATTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K104189-0022-110-D06C-CD50
TTGCATGGTCATCAAGGAGAAAATTATCTATGAAATCTATATCCAATTTAAATGGTGTCA
CATCAGTTTATGGATTCTATAACACCACGTGGAAACGTGCGACAGAATTGCTTTACTCAA
CTATTTTTTATATTACTATTATATTCAGTAATCCATTAGCTAAGTACTGAGTTTTGGTTG
AATCTTCTTGGTTGCTTTGGTATATCCTCTGGTTCTGTATCATTACAAGGTTGTCTTATT
CAGTAATCTTGGTTTATATCTCTGATTATGGATTAAGAGCTCAATACATGTTGGTCTAAT
AACCTAAAGATCATGTTGTTGCATTTACATTTTGGGGGTAATTAGGAGTTAATGATAGCT
AGATTATCAGCTAAACATTGGCTTTCAGTGTGCTGCTTTGTAGTGTGTTAGTTTCAGAGC
TTTGTGAGTTGATGATATTAACAATTTGTGGCAGGAACAGGGAAAGGAGGAGATTGAGTA
TTTCAGGCTTGGTGATCTCTGGGATTGTTATGATGAGATGAGTGCTTGGAATGAATTCGA
AGTGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCANGCG
TTATT
   
Sequence Name 45-K104190-0022-110-D06C-8409
GenBank Accession LN505517 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K104190-0022-110-D06C-8409
GCCCTTCGAATTCATTCCAGCACTCATCTCATCATAACAATCCCAGAGATCACCAAGCCT
GAAATACTCAATCTCCTCCTTTCCCTGTTCCTGCCACAAATTGTTAATATCATCAACTCA
CAAAGCTCTGAAACTAACACACTACAAAGCAGCACACTGAAAGCCAATGTTTAGCTGATA
ATCTAGCTATCATTAACTCCTAATTACCCCCAAAATGTAAATGCAACAACATGATCTTTA
GGTTATTAGACCAACATGTATTGAGCTCTTAATCCATAATCAGAGATATAAACCAAGATT
ACTGAATAAGACAACCTTGTAATGATACAGAACCAGAGGATATACCAAAGCAACCAAGAA
GATTCAACCAAAACTCAGTACTTAGCTAATGGATTACTGAATATAATAGTAATATAAAAA
ATAGTTGAGTAAAGCAATTCTGTCGCACGTTTCCACGTGGTGTTATAGAATCCATAAACT
GATGTGACACCATTTAAATTGGATATAGATTTCATAGATAATTTTTCTCCTTGATGGACC
ATGCAATTATTGTCTCTATAGCCCAAATCTCATCTGAAAACTCAC

Last Updated on 06.12.2018 16:11