Sequences from confirmation amplicon


Confirmation sequences for line 110F12 and hit At4g08620 are shown.
Line ID 110F12
Locus At4g08620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5503882
Primers used for PCR RK02 - 8474, show primer details
   
Sequence Name 20-K016437-022-110-F12C-8409
GenBank Accession LN494996 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K016437-022-110-F12C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCAGGATACTTCC
ATATACATTAATACTTCCATCAATAATTTTGTACCATTAAAAATATTTAATACTTAGAAT
AATTGATATTTTTTTTTGGTTAGAGAAAATTATTCTTACGTGACATCTCAATAATCACAA
ATCTGATTGCCGGCATGCCATATTCCTTAGCATTTTCTTGCTCCTCTCGCACCCATCTTG
ATGCCCTGTCACCAAATTTTCAATTATTTCATGACTTATACATATATACATCAAAATCAA
AACGTGTTAGCTCATAAAATTGCCCCGACGACTAATTCAATTAATATAAATATAATCGTT
GTGTTATGTTAGTGAGGTTTTAGTTTTCTTATGTTCTATTCAGAAAATTAAAATAAAAGT
AATAATTTCACAGAAAACGGCATAGTTTAC
   
Sequence Name 24-K016437-022-110-F12C-RK02
GenBank Accession LN496787 [GenBank]
Sequencing primer gene specific primer, RK02: 5'-TTGTAAACTATGCCGTTTTCTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K016437-022-110-F12C-rk02
ATTATTACTTTTATTTTAATTTTCTGAATAGAACATAAGAAAACTAAAACCTCACTAACA
TAACACAACGATTATATTTATATTAATTGAATTAGTCGTCGGGGCAATTTTATGAGCTAA
CACGTTTTGATTTTGATGTATATATGTATAAGTCATGAAATAATTGAAAATTTGGTGACA
GGGCATCAAGATGGGTGCGAGAGGAGCAAGAAAATGCTAAGGAATATGGCATGCCGGCAA
TCAGATTTGTGATTATTGAGATGTCACGTAAGAATAATTTTCTCTAACCAAAAAAAAATA
TCAATTATTCTAAGTATTAAATATTTTTAATGGTACAAAATTATTGATGGAAGTATTAAT
GTATATGGAAGTATCCTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11