Sequences from confirmation amplicon


Confirmation sequences for line 110H10 and hit At2g02250 are shown.
Line ID 110H10
Locus At2g02250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:594658
Primers used for PCR BJ23 - 8474, show primer details
   
Sequence Name 75-K104420-0022-110-H10C-BJ23
GenBank Accession LN513239 [GenBank]
Sequencing primer gene specific primer, BJ23: 5'-AGTGCAGTGCGTTTCAAATAATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K104420-0022-110-H10C-BJ23
TATAATTTTTGTACTATTTGAACTGTACCGCACTTTATTCGCAGTTGAACCGCACGTCTC
CATTCGGAGTCTAAGTAATCTGTGCAAAAGCATTGTAGATGATATGAAAACAAAAAAAGA
CAATTCCTTTATTGAAATATGTTCATGTGAGAATGATGAACATCCAAATCAAACAGTGCA
ATATCCAAAAATTAAAAACAGGTAGTGTTACCCATACAACAAAGTTTTTCACTTGCTTAT
ATAATGAACATCCAAATCAAACACAGTGCAATATCCAAAAACTAAAAACAGGTAGTGTTA
CCCATACAACAAAGTTTTTCACTTGATTATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATT
   
Sequence Name 91-K104421-0022-110-H10C-8409
GenBank Accession LN515120 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>91-K104421-0022-110-H10C-8409
GCCATTTACATTGAAATAATCAAGTGAAAAACTTTGTTGTATGGGTAACACTACCGTGTT
TTTAGTTTTTGGATATTGCACTGTGTTTGATTTGGATGTTCATTATATAAGCAAGTGAAA
AACTTTGTTGTATGGGTAACACTACCTGTTTTTAATTTTTGGATATTGCACTGTTTGATT
TGGATGTTCATCATTCTCACATGAACATATTTCAATAAAGGAATTGTCTTTTTTTGTTTT
CATATCATCTACAATGCTTTTGCACAGATTACTTAAACTCCGAATGGAGACGTGCGGTTC
AACTGCGAATAAAGTGCGGTACAGTTCAAATAGTAACAAAAATATATACATACATATATA
TGCAAAGAATTTCGTTATTATTTGAAACGCACTGCA

Last Updated on 06.12.2018 16:11