Sequences from confirmation amplicon


Confirmation sequences for line 111D07 and hit At4g32770 are shown.
Line ID 111D07
Locus At4g32770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15804714
Primers used for PCR RK03 - 3144, show primer details
   
Sequence Name 28-K016438-022-111-D07C-3144
GenBank Accession LN498628 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K016438-022-111-D07C-3144
GGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATT
TCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAA
CTCTGGTAAGTAGTTACGTGGCAAACCCCAAACGTAACGTATTTTTATTTTGTTAAATTA
ACAATTTCTCGTGTCTTGTTTTCAGTAATTTACGGAAATTTATTAGAGACAACATTTCAC
AAATTTGCTATTCTCTTGGATGTTGATTTGTAAAGCATGTCGTTTTATTGTAGGAATTTA
ACAGAAGAGTGTCCGAAGGGTTCCAAGCTACTCCATTTTGGCATCAAGGTCACATTTGCG
ATGATGGCCGGTAATTATATGATTCTATGCACAACAAGAATTCACTATATTATAAATATT
GGATATTGAGTATTTTTGTTGAAAATTTCTGTGTTTAAATCTGACTTGACTTGTTTTGTC
AGTACTGACTATGCGGAAACTGTGAAATCTGCTCGTTG
   
Sequence Name 32-K016438-022-111-D07C-RK03
GenBank Accession LN500366 [GenBank]
Sequencing primer gene specific primer, RK03: 5'-CTATACTCCCAACGAGCAGATTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K016438-022-111-D07C-rk03
AGTTTCCGCATAGTCAGTACTGACAAAACAAGTCAAGTCAGATTTAAACACAGAAATTTT
CAACAAAAATACTCAATATCCAATATTTATAATATAGTGAATTCTTGTTGTGCATAGAAT
CATATAATTACCGGCCATCATCGCAAATGTGACCTTGATGCCAAAATGGAGTAGCTTGGA
ACCCTTCGGACACTCTTCTGTTAAATTCCTACAATAAAACGACATGCTTTACAAATCAAC
ATCCAAGAGAATAGCAAATTTGTGAAATGTTGTCTCTAATAAATTTCCGTAAATTACTGA
AAACAAGACACGAGAAATTGTTAATTTAACAAAATAAAAATACGTTACGTTTGGGGTTTG
CCACGTAACTACTTACCAGAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGG
TACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGA
TAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTT
GAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGG
Primers used for PCR FI64 - 8474, show primer details
   
Sequence Name 09-K028243-022-111-D07C-8409
GenBank Accession LN490037 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K028243-022-111-D07C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAATCGTATTTTGAG
ATTTTTTTCCCTTTTAAAATATTTACAAAAATTCTGAAGAGAGCTGAAAAGAAAACAAAA
TAGAATACGTCAACAGCAGGATTCGAACCTGCGCGGGCAAAGCCCAATTGATTTCGAGTC
AATCTCCTTAACCACTCGGACATATTGACAATTTTGTTAGACAGACTCTTATACAAATTA
ATATATGCACGATCCAAAAGACTGATGATGATGAATGGACTAGATTTACTCTTCTTCATT
TGTCTCTTGTTTCTTTCTGTTAGACTAGATTTCAAAAACCCAAACGAATGGCAGGTTTTT
CTTGTTTACGACGAGCAACATAAGAACTAACAACAGTCGCAAACAAAACAAATCCTTCCT
TTCTACTTTTTCGGTAATT
   
Sequence Name 10-K028243-022-111-D07C-FI64
GenBank Accession LN490512 [GenBank]
Sequencing primer gene specific primer, FI64: 5'-TCAATGATGTCAATTACCGAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K028243-022-111-D07C-FI64
GTAGAAAGGAAGGATTTGTTTTGTTTGCGACTGTTGTTAGTTCTTATGTTGCTCGTCGTA
AACAAGAAAAACCTGCCATTCGTTTGGGTTTTTGAAATCTAGTCTAACAGAAAGAAACAA
GAGACAAATGAAGAAGAGTAAATCTAGTCCATTCATCATCATCAGTCTTTTGGATCGTGC
ATATATTAATTTGTATAAGAGTCTGTCTAACAAAATTGTCAATATGTCCGAGTGGTTAAG
GAGATTGACTCGAAATCAATTGGGCTTTGCCCGCGCAGGTTCGAATCCTGCTGTTGACGT
ATTCTATTTTGTTTTCTTTTCAGCTCTCTTCAGAATTTTTGTAAATATTTTAAAAGGGAA
AAAAATCTCAAAATACGATTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11