Sequences from confirmation amplicon


Confirmation sequences for line 111F03 and hit At1g07560 are shown.
Line ID 111F03
Locus At1g07560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2328193
Primers used for PCR KE09 - 8474, show primer details
   
Sequence Name 73-K107432-0022-111-F03C-KE09
GenBank Accession LN512910 [GenBank]
Sequencing primer gene specific primer, KE09: 5'-CAATAATTGTTGAGGAACACATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K107432-0022-111-F03C-KE09
TTATCCTAGCTTGTTTCTATGCTCTTGATAGCAAGGACTAACACACAATAACAGAATGAA
AACACTTAAGTATATACAAAAAAGTATATATATACTCAGATTTTGCTGACATACCGTCAT
TTGCATTAGTTTCAGACTGCGGAAACTCCACTGTTATAAAAGCCTCAATAGCATTGAGGA
GGGGAGGGAGAGTTGATTTTGGAGTTTTTATTANTTGCNAATGGNATGTCCCTCCTTCAC
ATTTAACTGGTGATGTATTGAATAGAGTACCCACTAGAAACTCGTCAGGACTAACAGGTC
CATAGTTAACATCTTGGCCTGCGCTAATGTTGAATTCCCTGGTATCATTTTCCCTTAAGG
ATTGGATCTCAGCGACGTGGAGGTATGCATAAACTAAGTCGTCAGGTGTTTCCAAATTCC
AAGAAATGGTTAATGGTGAACTCACATTTGCAGGTGTGGCTGCCGTAACCACTACGTCTT
CCGGTATGTCATAGCCGTTATCATCAGAAGTGTTGACCGTGAGACTTGTTCTTAACAGTC
TCCATTCCGGCATGAAAAATGGACTCCAAAGACGACAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCATCTATGACTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTCTTTTTCAATTCAAGAAAGTAGATGTCCG
   
Sequence Name 65-K107431-0022-111-F03C-8409
GenBank Accession LN511414 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K107431-0022-111-F03C-8409
TCATTGAATATATCCTGTCGTCTTTGGAGTCCATTTTTCATGCCGGAATGGAGACTGTTA
AGAACAAGTCTCACGGTCAACACTTCTGATGATAACGGCTATGACATACCGGAAGACGTA
GTGGTTACGGCAGCCACACCTGCAAATGTGAGTTCACCATTAACCATTTCTTGGAATTTG
GAAACACCTGACGACTTAGTTTATGCATACCTCCACGTCGCTGAGATCCAATCCTTAAGG
GAAAATGATACCAGGGAATTCAACATTAGCGCAGGCCAAGATGTTAACTATGGACCTGTT
AGTCCTGACGAGTTTCTAGTGGGTACTCTATTCAATACATCACCAGTTAAATGTGAAGGA
GGGACATGCCATTTGCAACTAATAAAAACTCCAAAATCAACTCTCCCTCCCCTCCTCAAT
GCTATTGAGGCTTTTATAACAGTGGAGTTTCCGCAGTCTGAAACTAATGCAAATGACGGT
ATGTCAGCAAAATCTGAGTATATATATACTTTTTTGTATATACTTAAGTGTTTTCATTCT
GTTATTGTGTGTTAGTCCTTGCTATCAAGAGCATAGAAACAAGCTATGGATTAAGTAGAA
TTAGTTGGCAAGGAGATCCATGTGTTCCTCAACAATTATTG

Last Updated on 06.12.2018 16:11