Sequences from confirmation amplicon


Confirmation sequences for line 112E10 and hit At2g02240 are shown.
Line ID 112E10
Locus At2g02240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:593199
Primers used for PCR 40vh - 8474, show primer details
   
Sequence Name 15-K117806-0022-112-E10C-40vh
GenBank Accession LN493162 [GenBank]
Sequencing primer gene specific primer, 40vh: 5'-AACACACATCAAGAAGCTCTGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117806-0022-112-E10C-40vh
TGTGCAATCATCATATTGCCAATTCCAAGGATTTAAAGAGGGGGAAAAAAGAGTTACCTA
GATTCAGGAATTGAAATCCATTGCCAATATTCAGGACTACTTCCCCATGTGATCCAAAGT
TCCTTTGAAGATAACATGATACACNCTTTTTCCACTCGCTTTTTCTAACCAAAAGCTCTT
TGATTTTTCGGAAAATAAAATAAAACATCAAAATAATCAGAATTACAACTACACCAAAAA
TGTTCTATCGTAGCAAGAATCAATTTTCTTAAATTAGGTTTTTAACAAATCTATGCGAAA
ACTCGTTGATTTAACACATGATTTTCAATTTGATCATTGTTCTCCAACGTATAAACCAAA
GAATAGATAGAATCATATAAATACCTTTTTGCCATCTTCGATTATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCACACGGTTTA
   
Sequence Name 15-K117784-0022-112-E10C-8409
GenBank Accession LN493161 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K117784-0022-112-E10C-8409
CATTTACAATTGAATAATCGAAGATGGCAAAAAGGTATTTATATGATTCTATCTATTCTT
TGGTTTATACGTTGGAGAACAATGATCAAATTGAAAATCATGTGTTAAATCAACGAGTTT
TCGCATAGATTTGTTAAAAACCTAATTTAAGAAAATTGATTCTTGCTACGATAGAACATT
TTTGGTGTAGTTGTAATTCTGATTATTTTGATGTTTTATTTTATTTTCCGAAAAATCAAA
GAGCTTTTGGTTAGAAAAAGCGAGTGGAAAAAGGTGTATCATGTTATCTTCAAAGGAACT
TTGGATCACATGGGGAAGTAGTCCTGAATATTGGCAATGGATTTCAATTCCTGAATCTAG
GTAACTCTTTTTTCCCCCTCTTTAAATCCTTGGAATTGGCAATATGATGATTTGCACACA
TTGTTGTTATAAAGATTCAACAAAATAGCAGAGCTTCTTGATGTGTGT

Last Updated on 06.12.2018 16:11