Sequences from confirmation amplicon


Confirmation sequences for line 113B03 and hit At2g37370 are shown.
Line ID 113B03
Locus At2g37370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15682859
Primers used for PCR 05uj - 8474, show primer details
   
Sequence Name 52-K117452-0022-113-B03C-05uj
GenBank Accession LN507937 [GenBank]
Sequencing primer gene specific primer, 05uj: 5'-GGAAAATTATTTCATGGCAAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K117452-0022-113-B03C-05uj
AAGGAACAAAAATGAAAACATAAATATATAAATCGTCTCTCCGGCGGAAAAAGGTCAAAA
AATATGCCGGGAGAGGTGAGAGTAATATACAAAACTTATTGGTTGGCTATAATATTATCT
ACACTTGGACCATATATTTCATATTTTAGGTTCAAAAAAGAAGAAATAAACTTACAATGT
CCTTTTATGGTTTAGTTAGATAGTAATTAGATATACTCTCCTCTTCTTCCATCATCTCCT
ACAACAAGACGAACATAATAAAATGTCACATTAACATCTACAATGTCTATCACCTAAAAA
AATGTTAATTTGAAAGCTTCATGTAGCTAAGTATGAGCTAAATATAGCTTACCTTTTCAA
AATTTGGGTCAACTACTTGTCTTTGAGGTCAGAGTTTCATCAAGAGCGTCGAATTCCCAG
TCATTGATTTCATCTGCCAAGACCCTGAAGTCCAGTTATATATACCCTGAATTCCCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATTCCC
   
Sequence Name 29-K117451-0022-113-B03C-8409
GenBank Accession LN499460 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K117451-0022-113-B03C-8409
GAATCAGGGTATATATAACTGGACTTCAGGGNTCTTGGCAGATGAAATCAATGACTGGGA
ATTCGACNGCTCTTGATGAAACTCTGACCTCAAAGACAAGTAGTTGACCCAAATTTTGAA
AAGGTAAGCTATATTTAGCTCATACTTAGCTACATGAAGCTTTCAAATTAACATTTTTTT
AGGTGATAGACATTGTAGATGTTAATGTGACATTTTATTATGTTCGTCTTGTTGTAGGAG
ATGATGGAAGAAGAGGAGAGTATATCTAATTACTATCTAACTAAACCATAAAAGGACATT
GTAAGTTTATTTCTTCTTTTTTGAACCTAAAATATGAAATATATGGTCCAAGTGTAGATA
ATATTATAGCCAACCAATAAGTTTTGTATATTACTCTCACCTCTCCCGGCATATTTTTTG
ACCTTTTTCCGCCGGAGAGACGATTTATATATTTATGTTTTCATTTTTGTTCCTTATTTT
TTTAAGTAGATGATATCAATGCATGATGACGGTAATACATGTTTTGCCT

Last Updated on 06.12.2018 16:11