Sequences from confirmation amplicon


Confirmation sequences for line 113F01 and hit At1g17260 are shown.
Line ID 113F01
Locus At1g17260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5904422
Primers used for PCR 19nu - 8474, show primer details
   
Sequence Name 44-K115005-0022-113-F01C-19nu
GenBank Accession LN505281 [GenBank]
Sequencing primer gene specific primer, 19nu: 5'-TGGATCCTGATACTTTCAACAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K115005-0022-113-F01C-19nu
CTTTTACTGTAACTCTTATTAGTACTTGAATTCTTAGTGGCAAAAAGGGTTTTGACCCTT
CTTGAGCTTGACAGGGTATATTGCCGTTGGAGGAGGTTTTTGAATACCTAAGAACATCGC
CTCAAGGGCTTTTATCTGGAGATGCTGAAGAGAGATTGAAGATATTTGGTCCTAACAGAC
TTGAAGAGAAACAGGTAATAAACATGACATTCTTCTTAGTGTTCCTGCCAATTGTCTCTA
TTATCTTGTGCTAACCGCCATTTAAAACAACAATGATACAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAACGTTAT
T
   
Sequence Name 12-K115004-0022-113-F01C-8409
GenBank Accession LN491699 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K115004-0022-113-F01C-8409
TCATTGAATATATCCTGTATCATTGTTGTTTAAATGGCGGTTAGCACAAGATAATAGAGA
CAATTGGCAGGAACACTAAGAAGAATGTCATGTTTATTACCTGTTTCTCTTCAAGTCTGT
TAGGACCAAATATCTTCAATCTCTCTTCAGCATCTCCAGATAAAAGCCCTTGAGGCGATG
TTCTTAGGTATTCAAAAACCTCCTCCAACGGCAATATACCCTGTCAAGCTCAAGAAGGGT
CAAAACCCTTTTTTGCCACTAAGAATTCAAGTACTAATAAGAGTATACAGTTAAAAGGAA
CATTTACCAAATCAATTCCTTTTCTGTTGAAAGTACCAGGATCC
Primers used for PCR 45ve - 3144, show primer details
   
Sequence Name 34-K117497-0022-113-F01C-45ve
GenBank Accession LN501576 [GenBank]
Sequencing primer gene specific primer, 45ve: 5'-ATCACCAGGTACCAAGATAGAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K117497-0022-113-F01C-45ve
AAATTTAAGGTAATATAGAAATTAAAGTGCAGCTAACTTTGCCGTTTCGGCTCATAGGAA
GGTTAAGAGCCATACTCTTGTTTTTAAAGCCAAGCGAGCCATAAGAGCTGCAGCAGCATT
CCCAGCATTGTTTTCTTCAAAGAAGCTGATTGTTGCGTTGATCAGCAAAAGGCAAACGAT
TCCAGTAAAGTCTTCCCAGTCAGGACCTAGACTCTGTAATACCATTTTCACCATGAGTTT
CAAGGTGCTTTTTCATAAACACTCATGGATGGAAAATGGTAGCAAAACTGTGGTTGTCTT
ACTTGACTATTAGCGAGGGCAATGGCCATCAATGCAGCAGCTTCCATAACCCATGACAAG
GGATTCCACATAAAACCTAAGAATTTCACAAATCTGTAGGAAGGGTCTCAAACACTGATA
GTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATG
AAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATC
CCTTACGTCAGTGGAGATA
   
Sequence Name 02-K117496-0022-113-F01C-3144
GenBank Accession LN487213 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117496-0022-113-F01C-3144
CTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAGACCCTTCCTA
CAGATTTGTGAAATTCTTAGGTTTTATGTGGAATCCCTTGTCATGGGTTATGGAAGCTGC
TGCATTGATGGCCATTGCCCTCGCTAATAGTCAAGTAAGACAACCACAGTTTTGCTACCA
TTTTCCATCCATGAGTGTTTATGAAAAAGCACCTTGAAACTCATGGTGAAAATGGTATTA
CAGAGTCTAGGTCCTGACTGGGAAGACTTTACTGGAATCGTTTGCCTTTTGCTGATCAAC
GCAACAATCAGCTTCTTTGAAGAAAACAATGCTGGGAATGCTGCTGCAGCTCTTATGGCT
CGCTTGGCTTTAAAAACAAGAGTATGGCTCTTAACCTTCCTATGAGCCGAAACGGCAAAG
TTAGCTGCACTTTAATTTCTATATTACCTTAAATTTGATTTAGATCCACTCTTATAGGTT
CTTAGAGATGGACAGTGGCAAGAACAAGATGCTTCTATCT

Last Updated on 06.12.2018 16:11