Sequences from confirmation amplicon


Confirmation sequences for line 113F01 and hit At3g18000 are shown.
Line ID 113F01
Locus At3g18000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6154253
Primers used for PCR 11qi - 8474, show primer details
   
Sequence Name 27-K115435-0022-113-F01C-11qi
GenBank Accession LN498529 [GenBank]
Sequencing primer gene specific primer, 11qi: 5'-AGCATCATAGCTTCAACAGTCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115435-0022-113-F01C-11qi
CCCCAAAAATAACAAAAAACAAATCAAATCAATTTTCACAGTTCTCTTCTCTTTCCATAA
ATCTCCAATTTCTATTAATATAAGCAATCAACGATAATCGAAGCATATGATCGGTGATCT
CACCGTACGATGCAGCCATTTCGGAAATGTCGTTTGTCGGGAAAAAACGAAGAAGAAGAT
CTGCCAAGAGAGGGGAGAGAGTAATTCAGTGGCCTTCGACGGCGAGTCTGGATCTAGAGA
AGCTTCGTGATCTGCGGTTGACGGATCGTCCTCTCTGCTGCATCAATTCGCACAACGTCG
AATCGGGGAAATGTAATTCAGATAAGGTTTCAGATCTGAGAGCGAAAAGAGTGTGAGAGT
GTGATGTTGCCTGCGCGCCCTTTTTTATAGGTGGAAAACGGTGGCGTTACCTTCGACTTC
GGCCTTTTTTACAGACTGTAAAATCAAATCAGTCGGTAATTAAATCAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTC
   
Sequence Name 11-K115434-0022-113-F01C-8409
GenBank Accession LN491253 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K115434-0022-113-F01C-8409
CGACTGATTCGATTTTACAGTCCTGTAAAAAAGGCCGAAGTCGAAGGTAACGNCCACCGT
TTTCCACCTATAAAAAAGGGCGCGCAGGCAACATCACACTCTCACACTCTTTTCGCTCTC
AGATCTGAAACCTTATCTGAATTACATTTCCCCGATTCGACGTTGTGCGAATTGATGCAG
CAGAAAAGAACGATCCGTCAACCGCAGATCACGAAGCTTCTCTAGATCCAGACTCGCCGT
CGAAGGCCACTGAATTACTCTCTCCCCTCTCTTGGCAGATCTTCTTCTTCGTTTTTTCCC
GACAAACGACATTTCCGAAATGGCTGCATCGTACGGTGAGATCACCGATCATATGCTTCG
ATTATCGTTGATTGCTTATATTAATAGAAATTGGAGATTTATGGAAAGAGAAGAGACTGT
GAAATTGATTTGATTTGTTTTTTGTTATTTTTGGGGTGAGTAGAAGAAGAGCGTGATATT
CAGAAGAATTACTGGATAGAGCATTCCGCTGATCTGACT

Last Updated on 06.12.2018 16:11