Sequences from confirmation amplicon


Confirmation sequences for line 113F11 and hit At4g30870 are shown.
Line ID 113F11
Locus At4g30870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15029329
Primers used for PCR HP11 - 8474, show primer details
   
Sequence Name 49-K017552-022-113-F11C-HP11
GenBank Accession LN506673 [GenBank]
Sequencing primer gene specific primer, HP11: 5'-TTTTACTTTGCTGGGTTGCTAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K017552-022-113-F11C-HP11
CCACTAATCCCTTCTGTATAAGAGTGGTCATGCAGCTCCATCCACTATACCACTCCCTCT
TAGAGTGTCCAAGGCCTGCTTTCCCTTTCCCTTTCTCTGGCCTAGTCAAAACTCACACGA
AATGGAAGAATAAGACATACAGCAGAGGTTGGGTACAAAACCAATATATTAGAAACTGGG
AACATCACGATTGATTCATACCCAACAGGAGCGTGTGATAGCCCATTCGCGTCAGCAGCA
TCAATAAGCTCTTGTTTACGCATGAATTCTTTCCCATTTGTAGTCCTTCTGGTAACACGA
AAAAACAAAAACGGTACTGAAGAAGGGTTTACAGAGGGGAAAGAAAGAGTATTTTGTGAG
TAACATAAACATGTAACTAACCTGTGCAACGTTATTAGTAATGCATATCCCACTGAATTT
CTTTGCGGAATGTACCGTTTTTTTTCCATTGGCCTTTTTCCCGGGACAGGTTAAAAATCA
GCATACATAAAGACATTACATTGATAATACATGTGTACNATGTGATATAGATACAGCCAA
CAGCCCAACACCACTACCAGCACGATTAT
   
Sequence Name 50-K017552-022-113-F11C-8409
GenBank Accession LN507021 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K017552-022-113-F11C-8409
GATCCATGTAGATTTCCCGGACATGAAGCCATCATACTCATTGCTGATTGCATAGGGGCT
TTGGCAAGTGGATGGTTAAGCTCATGAAAGGATATTTTGATACTGCTGTTGAAAGTTCTG
AGCAAGAAGATTTACCTGATAATCGTGCTGGTAGTGGTGTTGGGCTGTTGGCTGTATCTA
TATCACATTGTACACATGTATTATCAATGTAATGTCTTTATGTATGCTGATTTTTAACCT
GTCCAGGGAAAAAGGCCAATGGAAAAAAACGGTACATTCCGCAAAGAAATTCAGTGGGAT
ATGCATTACTAATAACGTTGCACAGGTTAGTTACATGTTTATGTTACTCACAAAATACTC
TTTCTTTCCCCTCTGTAAACCCTTCTTCAGTACCGTTTTTGTTTTTTCGTGTTACCAGAA
GGACTACAAATGGGAAAGAATTCATGCGTAAACAAGAGCTTATTGATGCTGCTGACGCGA
ATGGGCTATCACACGCTCCTGTTGGGTATGAATCAATCGTGATGTTCCCAGTTTCTAATA
TATTGGTTTTGTACCCAACCTCTGCTGTATGTCTTATTCTTCCATTTCGTGTGAGTTTTG
ACTAGGCCAGAGAAAGGGAAAGGGAAAGCAGGACTTGGACACTCTAAGAGGGAGTGGTAT
AGTGGATGGAGCTGCATGACCACTCTTATAC

Last Updated on 06.12.2018 16:11