Sequences from confirmation amplicon


Confirmation sequences for line 113G03 and hit At4g01920 are shown.
Line ID 113G03
Locus At4g01920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:829128
Primers used for PCR IH65 - 8474, show primer details
   
Sequence Name 81-K100855-0022-113-G03C-8409
GenBank Accession LN514031 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K100855-0022-113-G03C-8409
GCACATTCAAGAGAGCAGGAGTATTCATTCGTGGCTCCAAGTGTCTTCAAGATGATAGGA
AAAATACAATGCATCTTACACCAGCTGCAAAATGGACGAGATATACTATTATTGAGCACC
ACCTCATATGAAGTGCCCTCAATTTTTATTGTGCTTCTTCGCATGAGACTTGAAAGGTCT
CCAAGCACACACTTTGTATGCAAACTAGCCTGATGATCTTTACACGTATAGAACCATGTA
TGTGGATTTGTTTCTTTTTCACAAATGTCACACCAATATTTACCACTTGCCTTTTCGCCA
TAGCATAGTGAGAGAGGATGATCATGAACTCTATGCTTTACCATTTGTGGTAAAGTGGCG
CATCCAAAGCATAATAAAAATCCACAATTATCTTCAATGCAACTAAGCACAAAGTCCTGC
TCCATGTTGCAACCAGTGCACCATTGTACTTCGTCTGATGGAAAGTAATATAAGGGATGA
TTAGATTGACTTGGAT
   
Sequence Name 81-K100861-0022-113-G03C-IH65
GenBank Accession LN514032 [GenBank]
Sequencing primer gene specific primer, IH65: 5'-CCAAGTCATCCTAATCATCCCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K100861-0022-113-G03C-IH65
TGGTGCACTGGTTGCAACATGGAGCAGGACTTTGTGCTTAGTTGCATTGAAGATAATTGT
GGATTTTTATTATGCTTTGGATGCGCCACTTTACCACAAATGGTAAAGCATAGAGTTCAT
GATCATCCTCTCTCACTATGCTATGGCGAAAAGGCAAGTGGTAAATATTGGTGTGACATT
TGTGAAAAAGAAACAAATCCACATACATGGTTCTATACGTGTAAAGATCATCAGGCTAGT
TTGCATACAAAGTGTGTGCTTGGAGACCTTTCAAGTCTCATGCGAAGAAGCACAATAAAA
ATTGAGGGCACTTCATATGAGGTGGTGCTCAATAATAGTATATCTCGTCCATTTTGCAGC
TGGTGTAAGATGCATTGTATTTTTCCTATCATCTTGAAGACACTTGGAGCCACGAATGAA
TACTCCTGCTCTCTTGAATGTGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCC

Last Updated on 06.12.2018 16:11