Sequences from confirmation amplicon


Confirmation sequences for line 113G07 and hit At1g50260 are shown.
Line ID 113G07
Locus At1g50260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18619834
Primers used for PCR 27jn - 8474, show primer details
   
Sequence Name 52-K117671-0022-113-G07C-27jn
GenBank Accession LN507940 [GenBank]
Sequencing primer gene specific primer, 27jn: 5'-CACAGAATACGTACTCAACGCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K117671-0022-113-G07C-27jn
TTATTTACCTCTCCAGTACAATAGCCATATCAGCAAACCCAAGACGATCATTGACCTCAA
TTTGTAATACTTGCTCTCTAGGATTTGAAACAAGGAATTGAAAGTCCTGCAAAGTTAAAG
AATACACACTTATCTATTTACATTTCAATCCTTAAATAATCATAATTCACCACCAGATTG
TGAATGAACCATCCAAAGCAGTTCACCTGATTCCAAATTGGCTGACCAGGAGCCCCAATG
ACAGTAGTTTGACTGTTCCTTTTACTACGGATAACTTGATCCCCTAATCTGAGAATTGCA
TATGGATCCGTTTTACCTGTTACCAATTACCGAGGAAAACAAAACATCTCACCAATAGAC
TCAATTAGTATAGCTACTTTATTCCCAAGAAAGTCGACTGAACTTGCACAAAGAGGCTGA
TACTTCAGCAATAGATTGTCTGTCTATTCCACTTTTCCAACTATGAAGGAAACACTGACA
TGTAAAGGAACTATAGCTTACCAAAAAACATGTATCGAAGTTTCTGGGCGTCAACAAGAG
TAACAGATAGTTCCCCGACAAAATCCTTATTGCCTTCCTGCATTTCTCCTGATTTCAGGT
CTTCT
   
Sequence Name 04-K117670-0022-113-G07C-8409
GenBank Accession LN488091 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117670-0022-113-G07C-8409
ATTTCAATTGAATATATCCTGCATGTGGAACTTTAAGCTCCTCGACGTTTTGCAATAGTG
GGTTCCTTCTTCCCTCCTATTATTTTACCCAGCATCGTCTCTTACAGGTTCTTGACCAAA
CTGCTGACTGTGGATTTGCCTCGATTATTTGTCCGACCGAAGAAAATTGTCTTGGACTTT
CAGAAAGGAAAAGCTGTTGGACCTGTTTTAGAAGACCTGAAATCAGGAGAAATGCAGGAA
GGCAATAAGGATTTTGTCGGGGAACTATCTGTTACTCTTGTTGACGCCCAGAAACTTCGA
TACATGTTTTTTGGTAAGCTATAGTTCCTTTACATGTCAGTGTTTCCTTCATAGTTGGAA
AAGTGGAATAGACAGACAATCTATTGCTGAAGTATCAGCCTCTTTGTGCAAGTTCAGTCG
ACTTTCTTGGGAATAAAGTAGCTATACTAATTGAGTCTATTGGTGAGATGTTTTGTTTTC
CTCGGTAATTGGTAACAGGTAAAACGGATCCATATGCAATTCTCAGATTAGGGGATCAAG
TTATCCGTAGTAAAAGGAACAGTCAAACTACTGTCATTGGGGCTCCTGGTCAGCCAATTT
GGAATCAGGTGAACTGCTTTGGATGGTCATTCACAATCTGGTGGTGAATATGATTATTTA
AGGATGAAATGTAAATAGATAAGTGTGTATTCTTTACTTTGC

Last Updated on 06.12.2018 16:11