Sequences from confirmation amplicon


Confirmation sequences for line 115A12 and hit At3g25580 are shown.
Line ID 115A12
Locus At3g25580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9293945
Primers used for PCR 03fp - 8474, show primer details
   
Sequence Name 32-K111392-0022-115-A12C-03fp
GenBank Accession LN500636 [GenBank]
Sequencing primer gene specific primer, 03fp: 5'-TGTATTGTCTGATTCTTCCCTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K111392-0022-115-A12C-03fp
GACATGCTCTTGTGTGTTTGTCTCTTGAAGATATCGCTGTCGTGTTTCATCCGAGTGTGC
GGGATTTTTTGCTGCTGGGTTTAGCCTTTCTTCAAAAAGTTATTACTTGTTAGTTTTATT
GTTTTGGTCTTGATAAGAGATGTTAGGACAGACATGGTGCTTCTTGTCTATAGCCACTAG
ACCTATTTTAGCATAAGGTTAACGAAATTCTCTCTACATACCTTGTGGATTTGTTTACAT
TGCCTGATCTTTCCTGTGATCGCTGTCATGTTTCTTTGGAATGATTGATGTTTATAAATG
GAAAAATCTTTGTGCAGGTGATGGATAAACACATGAGCATATTGGCAAAGCAACACATTG
AGACACGTTTCGTGAAGATCCAAGCTGAGAAAAGTCCATTCTTGGCTGAGAGGCTTAAGA
TTGTTGTTCTTCCCACTCTTGCCCTCATTAAGAACACTAAAGTCGATGATTATGTGGTAT
ATAATAATAATGCTTAATCTAATCTCTTAGAATATGATTTATATGGATGAACTGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCAGCGTTATTATATAC
CACATNNTCNTCGACTTTAGTGTTCTTAATGAGGGCAAGAGTGGGAAGAACAACAATCTT
AAGCCTCTCAGCCAAGAATGGACTTTTCTCAGCTTGGATCTTCACGAAACGTGTCTCAAT
GTGTTGCTTTGCCAATATGCTCATGTGTTTATCCATCACCTGCACAAAGATTTTTCCATT
TATAAACATCAATCATTCCAAAGAAACATGACAGCGATCACAGGAAAGATCAGGCAATGT
AAACAAATC
   
Sequence Name 16-K111391-0022-115-A12C-8409
GenBank Accession LN493483 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K111391-0022-115-A12C-8409
CAGTTCTCCATATAATCATATTCTAAGAGATTAGATTAAGCATTATTATTATATACCACA
TAATCATCGACTTTAGTGTTCTTAATGAGGGCAAGAGTGGGAAGAACAACAATCTTAAGC
CTCTCAGCCAAGAATGGACTTTTCTCAGCTTGGATCTTCACGAAACGTGTCTCAATGTGT
TGCTTTGCCAATATGCTCATGTGTTTATCCATCACCTGCACAAAGATTTTTCCATTTATA
AACATCAATCATTCCAAAGAAACATGACAGCGATCACAGGAAAGATCAGGCAATGTAAAC
AAATCCACAAGGTATGTAGAGAGAATTTCGTTAACCTTATGCTAAAATAGGTCTAGTGGC
TATAGACAAGAAGCACCATGTCTGTCCTAACATCTCTTATCAAGACCAAAACAATAAAAC
TAACAAGTAATAACTTTTTGAAGAAAGGCTAAACCCAGCAGCAAAAAATCCCGCACACTC
GGATGAAACACGACAGCGATAATCTTCATAGAGACAAACACACAAGAGCATTGTTCCTAT
ATCACTTCCAAAGCTCTAAGAAATTCAAATATCACAGGGAAGAATCAGACAATAC

Last Updated on 06.12.2018 16:11