Sequences from confirmation amplicon


Confirmation sequences for line 115B08 and hit F4C21 are shown.
Line ID 115B08
Locus F4C21 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1442094
Primers used for PCR dl08 - 8474, show primer details
   
Sequence Name 24-K016388-022-115-B08C-dl08
GenBank Accession LN496786 [GenBank]
Sequencing primer gene specific primer, dl08: 5'-ACATTTCATCGCAATTACTCTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K016388-022-115-B08C-dl08
TTGCCTCTTATATGAAATATTATGTTCATTCACTTCCCAAGTTTCTTTTTTAATTTCCCG
TTTTCAAGGGTCACTTTGGGAGTAATTAGCTTTTGTTTGTTGACAATGGTACATGTAAGA
TCAGGTGTGTTTGCAATCAAAACAGGTATTTAAATACATTACTGAAAGATTAAATCAAAG
GCCCGTATAGCTCAGTGGTAGAGCGTCAGTCTTGTAAACTGAAGGTCCGTAGTTCGATCC
TGCGTGTGGGCACTTTTTATCCATTGCTTTTCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 23-K016388-022-115-B08C-8409
GenBank Accession LN496346 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K016388-022-115-B08C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAAAGCAATGGATAAAAAG
TGCCCACACGCAGGATCGAACTACGGACCTTCAGTTTACAAGACTGACGCTCTACCACTG
AGCTATACGGGCCTTTGATTTAATCTTTCAGTAATGTATTTAAATACCTGTTTTGATTGC
AAACACACCTGATCTTACATGTACCATTGTCAACAAACAAAAGCTAATTACTCCCAAAGT
GACCCTTGAAAACGGGAAATTAAAAAAGAAACTTGGGAAGTGAATGAACATAATATTTCA
TATAAGAGGCAAGAATTCACAGAGTAATTGCGAGGAAATG

Last Updated on 06.12.2018 16:11