Sequences from confirmation amplicon


Confirmation sequences for line 115C01 and hit At5g43900 are shown.
Line ID 115C01
Locus At5g43900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17663967
Primers used for PCR AS32 - 8474, show primer details
   
Sequence Name 13-K025673-022-115-C01C-8409
GenBank Accession LN491875 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K025673-022-115-C01C-8409
TAGATTTCCCGGACTCGAAGTACTTGATTGGTGTTCTTGATATTTATGGATTTGAGAGTT
TCAAGACAAACAGGTGCTTAGTGGTATGCTTGTTGTTTTCATAAATGGTTAGATGGGTTT
CTTTAATAAACTGGATCGTAGTCTGATATTTCACCATAATAGATGGGTCTGATCAGTTTG
ATAGTCAAGCTAGTGCTTGTTTCTAACATGGACAGTTGAGATTTCTTACTGCCTATCAAA
TTGTGAGCTTGTGAGGTTATCTGACTAACCTTGTTTTGTAAGGAGAGTACTGTTTGATTC
AACACATATAGTGCAACTGATGAATATTTCCGTACAAGATATCTCCATTGTTGACTGTTA
AAGAAATATTCATGACTGTTTCTTATTATTCTTTGGCCTAGTACTTACAATTGCAAGCTT
TAATGCAGTTTTGAGCAATTTTGCATCAATTTGACCAATGAAAAACTTCAACAGCATTTT
A
   
Sequence Name 14-K025673-022-115-C01C-AS32
GenBank Accession LN492345 [GenBank]
Sequencing primer gene specific primer, AS32: 5'-CAAACCTGATTAAAATGCTGTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K025673-022-115-C01C-AS32
TCATTGGTCAAATTGATGCAAAATTGCTCAAAACTGCATTAAAGCTTGCAATTGTAAGTA
CTAGGCCAAAGAATAATAAGAAACAGTCATGAATATTTCTTTAACAGTCAACAATGGAGA
TATCTTGTACGGAAATATTCATCAGTTGCACTATATGTGTTGAATCAAACAGTACTCTCC
TTACAAAACAAGGTTAGTCAGATAACCTCACAAGCTCACAATTTGATAGGCAGTAAGAAA
TCTCAACTGTCCATGTTAGAAACAAGCACTAGCTTGACTATCAAACTGATCAGACCCATC
TATTATGGTGAAATATCAGACTACGATCCAGTTTATTAAAGAAACCCATCTAACCATTTA
TGAAAACAACAAGCATACCACTAAGCACCTGTTTGTCTTGAAACTCTCAAATCCATAAAT
ATCAAGAACACCAATCAAGTACTTCGAGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTACA

Last Updated on 06.12.2018 16:11