Sequences from confirmation amplicon


Confirmation sequences for line 115C04 and hit At5g18550 are shown.
Line ID 115C04
Locus At5g18550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6162331
Primers used for PCR IL13 - 8474, show primer details
   
Sequence Name 24-K032414-022-115-C04C-IL13
GenBank Accession LN496889 [GenBank]
Sequencing primer gene specific primer, IL13: 5'-TGTTCCTGAGTTCATGAAAGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K032414-022-115-C04C-IL13
AGAGATAAGAGAGATATAATCTAAGAAGAATAGAGAGAGATTCTCTTCTCTCCTTTAAGA
TTTGTCTCTTTAACTTGAAGTCTTAGCCTCATTGCTAGCTGAATCACCTTTATTGGTCTC
AGCAGGCTCGGGATGGCTCACATCACTGGTCATAGAAGAGACTCCAGCAGCCACAGTTTC
TGAGCCACCAGTTGTTGTTGTTATAGGTTCAATAGAGCTGGAAGAGATAAGCTCAGTGCA
TTGGTCAGATGAGGATGATGGAGCTAATGTGCCGAGCGAGGAGGGGTAAGGTGCGACGGG
CATGTCCGTGAGTGAAGAAGGGGATGGGCTGTAGCTTAGTGAAGAAGAGCCCAAGGAGTG
GTCAAATTTGCAGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACTT
Primers used for PCR IL14 - 3144, show primer details
   
Sequence Name 26-K032415-022-115-C04C-IL14
GenBank Accession LN497806 [GenBank]
Sequencing primer gene specific primer, IL14: 5'-CATATGTTCAAAGCCCTTATGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K032415-022-115-C04C-IL14
TCGGGCTGGAACCCTTACAGGTATTGCTCCAAGAAACCTTTATTTGTTCTAACTTTTCTT
TCTCTTGTCTTAGTAAACCTCATTCCAATCATGTAGGCTTCTGTAAGTGCGATGCCTTCC
CCTGGGACTCAACCGTCAATGGGAACCAGCTCTGTTTATGGAATCACTCCGTTATCTCCC
TCAGCGCCTGCTTATCAATCCGGTCCATCTTCTACCGGTGTTTCAAATAAGGAACAAACT
TTTCCTCAGCGACCTGAACAACCTGAGTGTCAATACTTTATGAGAACCGGAGATTGTAAA
TTCGGAACCTCTTGCAGATTCCATCATCCTATGGAAGCAGCATCGCCAGAGGCAAGTACC
CTCAGCCACATTGGCCTCCCACTTCGTCCCGTAAGTCATATATACGCCATTAATCATGAA
ATTCTCATGATCAAGAGTAGAGTTTTAGTAATGACTTATGAGAAGTTAAAATGGAAAACA
CTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTC
CAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGC
GTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGG
AACGTCTTCTTTTTCCACGATGCTCCTCGT
   
Sequence Name 26-K032415-022-115-C04C-3144
GenBank Accession LN497805 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K032415-022-115-C04C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTTCC
ATTTTAACTTCTCATAAGTCATTACTAAAACTCTACTCTTGATCATGAGAATTTCATGAT
TAATGGCGTATATATGACTTACGGGACGAAGTGGGAGGCCAATGTGGCTGAGGGTACTTG
CCTCTGGCGATGCTGCTTCCATAGGATGATGGAATCTGCAAGAGGTTCCGAATTTACAAT
CTCCGGTTCTCATAAAGTATTGACACTCAGGTTGTTCAGGTCGCTGAGGAAAAGTTTGTT
CCTTATTTGAAACACCGGTAGAAGATGGACCGGATTGATAAGCAGGCGCTGAGGGAGATA
ACGGAGTGATTCCATAAACAGAGCTGGTTCCCATTGACGGTTGAGTCCCAGGGGAAGGCA
TCGCACTTACAGAAGCCTACATGATTGGAATGAGGTTTACTAAGACAAGAGAAAGAAAAG
TTAGAACAAATAAAGGTTTCTTTGGAGCAATACCTGGTAAGGGTTCCAGCCCGAGTATGG
AACCATACCAGGAGGAAGAACCATTTGACCGTAACCATAACG

Last Updated on 06.12.2018 16:11