Sequences from confirmation amplicon


Confirmation sequences for line 115E07 and hit At1g50870 are shown.
Line ID 115E07
Locus At1g50870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18855462
Primers used for PCR AC65 - 8474, show primer details
   
Sequence Name 05-K104226-0022-115-E07C-AC65
GenBank Accession LN488334 [GenBank]
Sequencing primer gene specific primer, AC65: 5'-TCCAAAGTCCACAATGTAATACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K104226-0022-115-E07C-AC65
TACACATGCTAACCTACCCTGATAAGGTATCAGCATGTTCGCAAAACTACCCGAAGGTAA
TGTTATCGTATCGAACTTTTCAGATCTGACCTCAAAACTCATTATAGTTGGATTGCTAAT
AAACCCCATCTGATCAATATAGGCTTGGTAATATATAACACCGTTAATGCATTGCCCATA
GTAACGAAGGATAGAACGATGCTTAAGGTTACTTTTGATCCTTCTCCATTTTTCTTGAGC
TGATCCCAGTGTTAAAACCCTACACTCATCGCTTGTGTTATCACGATTCATGCACATTAG
TTTGTGTTTACCTTCGACTGGATCGTATCCTAAAAAGACTGATAATCCCTTCCAGCTTTT
TTCCGGTTTAGGCAAGATTAAAAACTGTCTCATGGTAGGGTTCCAAATTATGGGTTTTGT
AGATATTCGAAAGCTGATTAACCCGTGGACAGATTCCGTGAAGGGGAAGCAACAATATTT
AGGGTATTTGATATGGTAACTATCCACATGCTGAGAATAAGAATGAGTATATNTTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAANAAGAAAGAGTAATTACCAATTTTTTTT
   
Sequence Name 21-K104227-0022-115-E07C-8409
GenBank Accession LN495640 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K104227-0022-115-E07C-8409
GAGCCATTTACAATTGAATATATACTCATTCTTATTCTCAGCATGTGGATAGTTACCATA
TCAAATACCCTAAATATTGTTGCTTCCCCTTCACGGAATCTGTCCACGGGTTAATCAGCT
TTCGAATATCTACAAAACCCATAATTTGGAACCCTACCATGAGACAGTTTTTAATCTTGC
CTAAACCGGAAAAAAGCTGGAAGGGATTATCAGTCTTTTTAGGATACGATCCAGTCGAAG
GTAAACACAAACTAATGTGCATGAATCGTGATAACACAAGCGATGAGTGTAGGGTTTTAA
CACTGGGATCAGCTCAAGAAAAATGGAGAAGGATCAAAAGTAACCTTAAGCATCGTTCTA
TCCTTCGTTACTATGGGCAATGCATTAACGGTGTTATATATTACCAAGCCTATATTGATC
AGATGGGGTTTATTAGCAATCCAACTATAATGAGTTTTGAGGTCAGATCTGAAAAGTTCG
ATACGATAACATTACCTTCGGGTAGTTTTGCGAACATGCTGATACCTTATCAGGGTAGGT
TAGCATGTGTTAATAATACCATGGACGATGTCAATGGTGGTATTACATTGTGAACTTTGG

Last Updated on 06.12.2018 16:11