Sequences from confirmation amplicon


Confirmation sequences for line 116H09 and hit At3g01420 are shown.
Line ID 116H09
Locus At3g01420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:161577
Primers used for PCR 07vf - 8474, show primer details
   
Sequence Name 44-K117914-0022-116-H09C-07vf
GenBank Accession LN505325 [GenBank]
Sequencing primer gene specific primer, 07vf: 5'-GAGATAGCTAAACCGTCTTCGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K117914-0022-116-H09C-07vf
GTCTTTGTAGTTCTTACTCTATCCAATGTTTTCGAGTCTCTTTCATAGATGACGCTCGAA
TCCCTAATTTATCAAATTATTTATGGTCATCATATATATACTTAAATGTATGCATGGATG
TGTGTTTGCGTCGTCCATATATATCATCATCTATAGAATAATTTATTTATTTTTCCTTTG
CGACAAATTTTGATCGTAACCGATCCCACAAAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTAATTAT
   
Sequence Name 04-K117913-0022-116-H09C-8409
GenBank Accession LN488099 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K117913-0022-116-H09C-8409
CATTTTTGTGGGATCGGTTACGATCAAAATTTGTCGCAAAGGAAAAATAAATAAATTATT
CTATAGATGATGATATATATGGACGACGCAAACACACATCCATGCATACATTTAAGTATA
TATATGATGACCATAAATAATTTGATAAATTAGGGATTCGAGCGTCATCTATGGAAGCAA
CTCGAAAACATTGGATAGAGTAAGAACTTACAAAGACGGGAAACTAAAGATATCGGAGGA
GACGGGTCTCCTTCTCCATGACGAAGACGGTTTAGCTATCTCAGACGGGAAACTAGTGAT
TTCGGATGATTTTGCTCTCCTTCACGATGACGAACACAGAAAAAAAAAAAAA

Last Updated on 06.12.2018 16:11