Sequences from confirmation amplicon


Confirmation sequences for line 117F04 and hit At3g27210 are shown.
Line ID 117F04
Locus At3g27210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10046576
Primers used for PCR 17ra - 8474, show primer details
   
Sequence Name 76-K115895-0022-117-F04C-17ra
GenBank Accession LN513451 [GenBank]
Sequencing primer gene specific primer, 17ra: 5'-AATTCTTGCCTAAACCCTTCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K115895-0022-117-F04C-17ra
ACCAGATCAGAAACTGATTAGCAACACTTTTTCATATTTTTGTCAGATCTGGGTCACAGT
TAAAGTAGACAACTTTTCAAATCATAATTCACCAAAATAGCTCACTTTTACTTTTTTCTT
TCTGCTGTGAGAAAAATCTCTATGATCACGGGAAAGTTTTTGTCTTTTACGGCATAAAAA
TCTATTAATGGAAGAAATCAAATTTGATATATGATCTTATCAGATAAGAAAAAAATAACC
TATCTACGTTAACAATATGGATGAGTATGTTATCAAACCTATCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCACACAG
TTATA
   
Sequence Name 46-K115894-0022-117-F04C-8409
GenBank Accession LN505965 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K115894-0022-117-F04C-8409
ATTGAATATATCCTGATAGGTTTGATAACATACTCATCCATATTGTTAACGTAGATAGGT
TATTTTTTTCTTATCTGATAAGATCATATATCAAATTTGATTTCTTCCATTAATAGATTT
TTATGCCGTAAAAGACAAAAACTTTCCCGTGATCATAGAGATTTTTCTCACAGCAGAAAG
AAAAAAGTAAAAGTGAGCTATTTTGGTGAATTATGATTTGAAAAGTTGTCTACTTTAACT
GTGACCCAGATCTGACAAAAATATGAAAAAGTGTTGCTAATCAGTCTTCTTGATCTGGGT
TCCATTGTTTTTCACGTATTAATTTGAAGGGTTTAGCCAAGAAT

Last Updated on 06.12.2018 16:11