Sequences from confirmation amplicon


Confirmation sequences for line 117G12 and hit At4g26790 are shown.
Line ID 117G12
Locus At4g26790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13488760
Primers used for PCR KS74 - 8474, show primer details
   
Sequence Name 33-K101501-0022-117-G12C-8409
GenBank Accession LN500932 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K101501-0022-117-G12C-8409
CCATTTACATGTAGATTTCAGGATGGTATATATCTTTGGATTCCGTTCAGATCTCTGTTC
AACTGGAAGACTTTCTCTTCCATCTTGATGTTGAAATCTCTAGCCACAATGTTGTATTCT
TCAATACATTTGCTTCCATAAAAGAGCTGAGTTGTTCTTTCCAGGGGTAAGCATCCAAAA
GGAGAGAGTCCCGACAAAGACATCTTCCGAGCTCCGAGTCTGTATATATCTGTCACGAAA
TCTGCTGCTATTCCTATCAGGAAATATTGGTACTCGTTCACTGAGTACTTCCGTAACTTG
CGGGGAAGAAGATAGTAATTCTCCAGGAAATCGTTGGTTCCGATACTAATCAGGTAAAGT
GATTCACTAATAATCTCATTAGCTTTCTCTTCACCCAAGTAGCTTCTTAGCCTGGTTTGG
TATTCTTTGTAGTATTCCACTTCCTTCCATAGCGGCNTTACAGACTTTAGAAAT
   
Sequence Name 33-K101505-0022-117-G12C-KS74
GenBank Accession LN500933 [GenBank]
Sequencing primer gene specific primer, KS74: 5'-GATTTCTAAAGTCTGTAATGCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K101505-0022-117-G12C-KS74
GATACCAACCAGGCTAAGAAGCTACTTGGGTGAAGAGAAAGCTAATGAGATTATTAGTGA
ATCACTTTACCTGATTAGTATCGGAACCAACGATTTCCTGGAGAATTACTATCTTCTTCC
CCGCAAGTTACGGAAGTACTCAGTGAACGAGTACCAATATTTCCTGATAGGAATAGCAGC
AGATTTCGTGACAGATATATACAGACTCGGAGCTCGGAAGATGTCTTTGTCGGGACTCTC
TCCTTTTGGATGCTTACCCCTGGAAAGAACAACTCAGCTCTNTTATGGAAGCAAATGTAT
TGAAGAATACAACATTGTGGCTAGAGATTTCAACATCAAGATGGAAGAGAAAGTCTTCCA
GTTGAACAGAGATCTGAACGGAATCCAAAGATATATACCATCCTGAAATCTACATGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCNAAAATGTAG

Last Updated on 06.12.2018 16:11