Sequences from confirmation amplicon


Confirmation sequences for line 117H11 and hit At5g22930 are shown.
Line ID 117H11
Locus At5g22930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7668629
Primers used for PCR IH66 - 8474, show primer details
   
Sequence Name 83-K100855-0022-117-H11C-8409
GenBank Accession LN514275 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K100855-0022-117-H11C-8409
ATTTACATTGAATATATACTCAACAACTAAGATCCAACAAGAGCTTCTCATCTTCTGATT
GCGAGGAGAGCATTATGTCACCAACTGATCATATCATGAATCCACCACCATGTCAGCTTG
AAGAAGTCTCAGGAACTGAGATTATCATGGATCCATTGTTGCAAGACAAGCCACTGCCTG
AGAAGGGTAAGCTTTTACAAGCTGTTATTAAGGCAGGGCCTTTACTTCAGACACTGCTCT
TAGCTGGTCCATTACCTCAATGGCGCCATCCGCCACCGCCTCTCAAGAGCTTCGAGATCC
CTCCGGTCACAGTCCAATGCCCAATTGTCAACAATGGATGTGGGAAGTTCAACAGGAAAA
GAGTATTCTCAGATGGATCCTATTCAGAAGCTAAGTATCAGAAAGTTCTTCTCCAATGAC
CGGGCAATGAAAACATTTCTGGCTACTGATCTTTTATCTTACTCAAGTAGTACTAATAGT
CATCTAATATACCATTACATACATAGGTCAATTCCAGATTAATCTCACTTCTAGTGTTCA
AGTGAGAGTGATCTTTTTGTACATTAGGGCTTG
   
Sequence Name 83-K100861-0022-117-H11C-IH66
GenBank Accession LN514276 [GenBank]
Sequencing primer gene specific primer, IH66: 5'-AGCATAACCAAAAGCCCTAATGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K100861-0022-117-H11C-IH66
GAGATTAATCTGGAATATGACCTATGTATGTAATGGTATATTAGATGACTATTAGTACTA
CTTGAGTAAGATAAAAGATCAGTAGCCAGAAATGTTTTCATTGCCCGGTCATTGGAGAAG
AACTTTCTGATACTTAGCTTCTGAATAGGATCCATCTGAGAATACTCTTTTCCTGTTGAA
CTTCCCACATCCATTGTTGACAATTGGGCATTGGACTGTGACCGGAGGGATCTCGAAGCT
CTTGAGAGGCGGTGGCGGATGGCGCCATTGAGGTAATGGACCAGCTAAGAGCAGTGTCTG
AAGTAAAGGCCCTGCCTTAATAACAGCTTGTAAAAGCTTACCCTTCTCAGGCAGTGGCTT
GTCTTGCAACAATGGATCCATGATAATCTCAGTTCCTGAGACTTCTTCAAGCTGACATGG
TGGTGGATTCATGATATGATCAGTTGGTGACATAATGCTCTCCTCGCAATCAGAAGATGA
GAAGCTCTTGTTGGATCTTAGTTGTTGAGTATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11