Sequences from confirmation amplicon


Confirmation sequences for line 118H08 and hit At1g23170 are shown.
Line ID 118H08
Locus At1g23170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8214462
Primers used for PCR 44vi - 8474, show primer details
   
Sequence Name 44-K118077-0022-118-H08C-44vi
GenBank Accession LN505329 [GenBank]
Sequencing primer gene specific primer, 44vi: 5'-GCGTAAGAAACCTGATGCTTTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K118077-0022-118-H08C-44vi
CAGGATAAGCTTTCAGTTACAGTTTGGATGATGGCTCAGGTGTGTGAAGGTCTATTCATA
ATAGTATAAACGAAGAACCAAAATGTATTGTTTTGATGATTGAGATGATTCTGAAATTTG
CTTTCTCTTAACTGCCAACTATGCACAGGCCTCCCAAGGTGATATATCAGTAGGCTTGTA
CTCGTGGGCTCACAACTTGTTACCGGTGGTCAGTAGCAAGAGTTGCAATCCTCAGTCAAG
GGATCTCATTCTTCAGTTGGTTGAGAGGTGAGTTAAGTTTTTCCTTTGATGTCCTGTTTT
CTATGTGATGATTATCTAATCTTGGTTATTGTGTTTTTATTGCAGGATCTTGTCCAACCC
GAAGGCCCGGACCATACTTGTAAATGGGGCTGTTAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 19-K118076-0022-118-H08C-8409
GenBank Accession LN494951 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118076-0022-118-H08C-8409
TTGAAATATCCTAACAGCCCCATTTACAAGTATGGTCCGGGCCTTCGGGTTGGACAAGAT
CCTGCAATAAAAACACAATAACCAAGATTAGATAATCATCACATAGAAAACAGGACATCA
AAGGAAAAACTTAACTCACCTCTCAACCAACTGAAGAATGAGATCCCTTGACTGAGGATT
GCAACTCTTGCTACTGACCACCGGTAACAAGTTGTGAGCCCACGAGTACAAGCCTACTGA
TATATCACCTTGGGAGGCCTGTGCATAGTTGGCAGTTAAGAGAAAGCAAATTTCAGAATC
ATCTCAATCATCAAAACAATACATTTTGGTTCTTCGTTTATACTATTATGAATAGACCTT
CACACACCTGAGCCATCATCCAAACTGTAACTGGAAGCTTATCCTGTCCCTGATACTTGG
GATTCTCCCTCAAAGTTGGCAATATATTAGTTAAAGCATC

Last Updated on 06.12.2018 16:11