Sequences from confirmation amplicon


Confirmation sequences for line 119A09 and hit At1g75920 are shown.
Line ID 119A09
Locus At1g75920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28506867
Primers used for PCR AC56 - 3144, show primer details
   
Sequence Name 63-K104216-0022-119-A09C-AC56
GenBank Accession LN510982 [GenBank]
Sequencing primer gene specific primer, AC56: 5'-ATGTCAAGACTTATTTTTGGTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K104216-0022-119-A09C-AC56
TCTCTGAAGATAAACAAAAAATTGAAAAGCGGAATTAAAAGTTGGAGAGGAGCATCAGAT
TTTAGGGGTGCTAGGTTTGTCTATGTCGACATGTACAACTCTCTTATGGATGTTATCAAC
AATCATAGGAAATACGGTACGTAAATACGTAAATCTCCTTTTTATAAAAATATATGCATT
ACCAAGATCAATACAAAGGAACCACATATTTAGTGATGCTTAGACATTTGAAATCTCAGG
AGCGACCATAATAGTTAGGTATTGTGTACTTGTATGGTTTCAGGATTTACCTATTGGAAT
CAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGT
CCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGA
TTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCA
   
Sequence Name 23-K104217-0022-119-A09C-3144
GenBank Accession LN496535 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K104217-0022-119-A09C-3144
ACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAG
GGTACCCGGAGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATT
CCAATAGGTAAATCCTGAAACCATACAAGTACACAATACCTAACTATTATGGTCGCTCCT
GAGATTTCAAATGTCTAAGCATCACTAAATATGTGGTTCCTTTGTATTGATCTTGGTAAT
GCATATATTTTTATAAAAAGGAGATTTACGTATTTACGTACCGTATTTCCTATGATTGTT
GATAACATCCATAAGAGAGTTGTACATGTCGACATAGACGAACCTAGCACCCCTAAAATC
TGATGCTCCTCTCCAACTTTTAATTCCGCTTTTCAATTTTTTGTTGTAATCTTCAGAGAT
TGTGTTTGCCAAGAAATTACACCACACGAAGAACCCACCAAAAAT

Last Updated on 06.12.2018 16:11