Sequences from confirmation amplicon


Confirmation sequences for line 119E09 and hit At3g22270 are shown.
Line ID 119E09
Locus At3g22270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7875323
Primers used for PCR 03wm - 8474, show primer details
   
Sequence Name 73-K118585-0022-119-E09C-03wm
GenBank Accession LN512976 [GenBank]
Sequencing primer gene specific primer, 03wm: 5'-TGCTTAGTTTTCGATGTTGTGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K118585-0022-119-E09C-03wm
AAACTTGTCTTTGAGATGTTGTGTTAATCCCTTGAATTGTTAAAGTCTAGATTTTTATGG
TCATGTGTTTGTAATGTTGGATTCTGAAACAGCTGTTTTTGTATCTGCAGATAAAAACAG
TACACTCTTTGATGCATCACAATATGAGTTTTTTGGACAAAATCTGGATGATATGGAACT
TGGGGGTCTTGATGATGATGGAGTAATCGCTCCTGTTCTTGGACATGCTGATGATGATGA
GTATCACTTGTTTGATAAAGGAGAGGTAACATGCTTTCTCTGTTGAAAGCTAACATCTTG
ATAATGATGCATGTAGTAAGTAATGGTTGCAGATGTTTTACTAATAGATATGCATGTTGG
TTCCAGGGTGCAGGTTTAGGATCACTATCTGACATGGATGATCTTGCGACAACTTTCGCA
AAGGTTAGCATAATTTTTATTTACAAACACTATGATGACAATCTTAATATCTTACCTACC
ACCATGTCATGGTTGCATGTGATAAACTGATAATCAATCATCTTCTTTTTGTTTCATGAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCA
   
Sequence Name 43-K118584-0022-119-E09C-8409
GenBank Accession LN505006 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K118584-0022-119-E09C-8409
TTGAATATCATGAAACAAAAAGAAGATGATTGATTATCAGTTTATCACATGCAACCATGA
CATGGTGGTAGGTAAGATATTAAGATTGTCATCATAGTGTTTGTAAATAAAAATTATGCT
AACCTTTGCGAAAGTTGTCGCAAGATCATCCATGTCAGATAGTGATCCTAAACCTGCACC
CTGGAACCAACATGCATATCTATTAGTAAAACATCTGCAACCATTACTTACTACATGCAT
CATTATCAAGATGTTAGCTTTCAACAGAGAAAGCATGTTACCTCTCCTTTATCAAACAAG
TGATACTCATCATCATCAGCATGTCCAAGAACAGGAGCGATTACTCCATCATCATCAAGA
CCCCCAAGTTCCATATCATCCAGATTTTGTCCAAAAAACTCATATTGTGATGCATCAAAG
AGTGTACTGTTTTTATCTGCAGATACAAAAACAGCTGTTTCAGAATCCAACATTACAAAC
ACATGACCATAAAAATCTAGACTTTAACAATTCAAGGGATTAACACAACATCTCTAAAGA
CAAGTTTTTTAGTATCCAAACTGGTCTATCATTTGTGCATTGGAACACAACATCGAAAAC
TAAGC

Last Updated on 06.12.2018 16:11