Sequences from confirmation amplicon


Confirmation sequences for line 120B05 and hit At5g62470 are shown.
Line ID 120B05
Locus At5g62470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25080512
Primers used for PCR M96a - 8474, show primer details
   
Sequence Name 35-K014706-022-120-B05C-8409
GenBank Accession LN501614 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K014706-022-120-B05C-8409
TCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATAGAACAAAAATGGC
TTCATGTAGATTATCTACATTTTTATTTAATATCTCTAATTACCATTTAATCCCTTTTTC
CTTGAGTTAATTGTTTTGTTAATATGGTGTTTTAGGTTTGAGAAGATGTAGCAAGAGCTG
CAGATTGAGATGGACTAATTATCTTCGACCCGGTATTAAGCGTGGAAATTTTACTGAGCA
TGAAGAGAAGACAATTGTTCATCTTCAAGCCCTTTTAGGCAACAGGTATATTTTAATGCA
CATGATCCATATTTTTCCAAAAAGTTACCTTTCTTGGGTATAAGATCTAAATATGTTTTT
CTTTGTATATAGATGGGCAGCCATAGCATCATACCTTCCAGAAAGGACAGACAATGATAT
AAAGAACTATTGGAACACTCACTTGAAGAAGAAGCTCAAAAAGATTAATGAATC
   
Sequence Name 36-K014706-022-120-B05C-M96a
GenBank Accession LN502022 [GenBank]
Sequencing primer gene specific primer, M96a: 5'-GACAAGGCCTCACAAAGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K014706-022-120-B05C-M96a
TGTTTCGCCTGTTAATGCCTGTCTGAAGCCTTCTTTCCATTGACCTTTGTTAGTGCTTTG
ATGGTTCTTTTGTGAACTAGTGCTTGATGAAGAGACACCATCATTATCTTCTTCACCAGA
TTCATTAATCTTTTTGAGCTTCTTCTTCAAGTGAGTGTTCCAATAGTTCTTTATATCATT
GTCTGTCCTTTCTGGAAGGGATGATGCTATGGCTGCCCATCTATATACAAAGAAAAACAT
ATTTAGATCTTATACCCCAAGAAAGGTAACTTTTTGGAAAAATATGGATCATGTGCATTA
AAATATACCTGTTGCCTAAAAGGGCTTGAAGATGAACAATTGTCTTCTCTTCATGCTCAG
TAAAATTTCCACGCTTAATACCGGGTCGAAGATAATTAGTCCATCTCAATCTGCAGCTCT
TGCTACATCTTCTCAAACCTAAAACACCATATTAACAAAACAATTAACTCAAGGAAAAAG
GGGATTAAATGGTAATTAGAGATATTAAATAAAAATGTCGATAATCTACATGAAGCCCAT
TTTTGGTCTAT

Last Updated on 06.12.2018 16:11