Sequences from confirmation amplicon


Confirmation sequences for line 120C04 and hit At2g03160 are shown.
Line ID 120C04
Locus At2g03160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:959651
Primers used for PCR CK01 - 8474, show primer details
   
Sequence Name 21-K017715-022-120-C04C-CK01
GenBank Accession LN495459 [GenBank]
Sequencing primer gene specific primer, CK01: 5'-GTTTTGGAACTTGTTGTTTTCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K017715-022-120-C04C-CK01
CTCAAAAGTCCAAGCGTTTTCATTGCGAATTGCTTCTTCTTCTTCAGGTGTGAAATCATT
CTCGATGTTAAAGAGTTCTCGAACTTCCTCTGGCGTCATATCTTTTATGTAATCTGCAAT
GGTCTTGCTACAGAGATCAAAAAGACCTTGGACGTTGAGATAGTTAGCAGCCAGAATAAT
GTCGAAGATCGTCTTAATATCGAAATCCTTCATGAACTTTGCATCCCACTCATTAAGCTT
CTCCTTCTTATCCTCAGCTTCATCATCTCCTTCAGTTGATTCAGGTACGGCCACATCATC
AGGCTTCTTCTTCGCCTCCTCAACTGTTTTATCTCCTTTCGTCGATGATTCATGCGTCTC
CACCACGTCATCATCGTCTTCAACGTGTTTCTTGCAGTACTCGATAACCTTGGCGAGAAT
CTCTCCGGTAACGTTTGGAATAGGGATTTTGTTTGTAGCACAGTCGTCTTCGATAATGTG
TCCTACGATCTGCAGTTTTCTTGCCACCACTTCTTCAACCTTGAAAGATTCACCATCGGA
GCTTGTCAACACAATCTTTTTCGATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
   
Sequence Name 22-K017715-022-120-C04C-8409
GenBank Accession LN495904 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K017715-022-120-C04C-8409
TCCATGTAGATTTCCCGGACATGAAGCCATTTACAATCGAAAAAGATTGTGTTGACAAGC
TCCGATGGTGAATCTTTCAAGGTTGAAGAAGTGGTGGCAAGAAAACTGCAGATCGTAGGA
CACATTATCGAAGACGACTGTGCTACAAACAAAATCCCTATTCCAAACGTTACCGGAGAG
ATTCTCGCCAAGGTTATCGAGTACTGCAAGAAACACGTTGAAGACGATGATGACGTGGTG
GAGACGCATGAATCATCGACGAAAGGAGATAAAACAGTTGAGGAGGCGAAGAAGAAGCCT
GATGATGTGGCCGTACCTGAATCAACTGAAGGAGATGATGAAGCTGAGGATAAGAAGGAG

Last Updated on 06.12.2018 16:11