Sequences from confirmation amplicon


Confirmation sequences for line 120H11 and hit At5g43370 are shown.
Line ID 120H11
Locus At5g43370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17416465
Primers used for PCR 13lf - 3144, show primer details
   
Sequence Name 68-K113920-0022-120-H11C-35St
GenBank Accession LN512058 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K113920-0022-120-H11C-35St
TTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGACGATAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTATAAACAAATCTTG
ATATTAATTCATAAACAAAGGGTGGTCATGACACCCTTGGCTTCGTTGCCAAAAGAGAGG
CCAGAAGCGACAGAGCATAAGATCATCATGATCAAAGTAAGACCGTACACTTTTTTCCGT
CCGAGTTTGTCACCGAGCCAACCGAAGAAGAGTTGACCAGAAAGGGTTCCACAAAGGGCC
ACACCATTGACAGCGGCCGCAACATGAGGGGGAAGTGAGCCAGGCTTCGCTGACTCCGGA
TTGAAGTAGTAGATGCGGCCAAGGAGCTTTGTTACCAAGGACACGCAAAAAAGATCGTAC
GCATCGGTAAAGAAACCCATACCGGCGATGACAATCGCCGTGAAATGATAAAGTTGCGTC
TTCGCAACATCGAGTGCCTTTAGCACTCCTAGTTGTTGTTCAGCCATCTAAGCCTCTCTT
GTCTTTCCTGCTTCATGAAGTAAAAATAAATAT
   
Sequence Name 58-K113919-0022-120-H11C-13lf
GenBank Accession LN509765 [GenBank]
Sequencing primer gene specific primer, 13lf: 5'-ATTTATTTTTACTTCATGAAGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K113919-0022-120-H11C-13lf
CACACTAGGAGTGCTAAAGGCACTCGATGTTGCGAAGACGCAACTTTATCATTTCACGGC
GATTGTCATCGCCGGTATGGGTTTCTTTACCGATGCGTACGATCTTTTTTGCGTGTCCTT
GGTAACAAAGCTCCTTGGCCGCATCTACTACTTCAATCCGGAGTCAGCGAAGCCTGGCTC
ACTTCCCCCTCATGTTGCGGCCGCTGTCAATGGTGTGGCCCTTTGTGGAACCCTTTCTGG
TCAACTCTTCTTCGGTTGGCTCGGTGACAAACTCGGACGGAAAAAAGTGTACGGTCTTAC
TTTGATCATGATGATCTTATGCTCTGTCGCTTCTGGCCTCTCTTTTGGCAACGAAGCCAA
GGGTGTCATGACCACCCTTTGTTTATGAATTAATATCAAGATTTGTTTATAAACACTGAT
AGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAAT
GAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCAT
CCCTTACGTCAGTGGAGATATCACAACAATCCA

Last Updated on 06.12.2018 16:11