Sequences from confirmation amplicon


Confirmation sequences for line 121A12 and hit At2g44840 are shown.
Line ID 121A12
Locus At2g44840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18495733
Primers used for PCR CM01 - 8474, show primer details
   
Sequence Name 13-K019821-022-121-A12C-CM01
GenBank Accession LN491833 [GenBank]
Sequencing primer gene specific primer, CM01: 5'-ACATCCCACATTGATTGTTACTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K019821-022-121-A12C-CM01
ATTTGAAATCTTTTTTTTCCTAGTTATATAAGAAAATAGATTATTATAGAAGTTTTACTA
TGGAACCGAATTTAGAAGAAAAAAACAAACGTCAATATTTAATTTCCATCACATTACATA
TGATCATGATCAAAACCTGTTGAGTCATCGATAGATATTTCATACAGCAAAACTCGTTGG
ACAAATTTTGCTTAATCTTAAAACTAATTATATCCGATTATCAGAATAAGAACATTCTGA
TTGGTCCATTAATAAACTACCATCGAAGTAAAACTGATCCACCGTGAAATCCAACTCCGG
TACAACCAAACTAGACTCGCCGTCATCCACGTGGCTTTCCCTCTTCTGCTCCGACGTTAA
CTGATCGGAGACTGACGGTTCCGGCGAGCGACGGCGAGGCCTAATCCTAACCGGCTCATA
CTTACAAGAACCAATCAAATGCGGAAAATTCAGCTTAGCTTTCGATCCTCTGAGCTGAAA
CGCCGCTCGGTCGTACGCCACCGCCGCGTCCTCCGGCGTCTCGTAAGTCCCGAGCCAAAC
CCTAGCTCCGTTCTTCTTCGGATCCCTAATCTCCGCCGCGAATTTCCCCCACGGCTATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
   
Sequence Name 14-K019821-022-121-A12C-8409
GenBank Accession LN492302 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K019821-022-121-A12C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAGCCGTGGGGGAAATTCGCGGC
GGAGATTAGGGATCCGAAGAAGAACGGAGCTAGGGTTTGGCTCGGGACTTACGAGACGCC
GGAGGACGCGGCGGTGGCGTACGACCGAGCGGCGTTTCAGCTCAGAGGATCGAAAGCTAA
GCTGAATTTTCCGCATTTGATTGGTTCTTGTAAGTATGAGCCGGTTAGGATTAGGCCTCG
CCGTCGCTCGCCGGAACCGTCAGTCTCCGATCAGTTAACGTCGGAGCAGAAGAGGGAAAG
CCACGTGGATGACGGCGAGTCTAGTTTGGTTGTACCGGAGTTGGATTTCACGGTGGATCA
GTTTTACTTCGATGGTAGTTTATTAATGGACCAATCAGAATGTTCTTATTCTGATAATCG
GATATAATTAGTTTTAAGATTAAGCAAAATTTGTCCAACGAGTTTTGCTGTATGAAATAT
CTATCGATGACTCAACAGGTTTTGATCATGATCATATGTAATGTGATGGAAATTAAATAT
TGACGTTTGTTTTTTTCTTCTAAATTCGGTTCCATAGTAAAACTTCTATAATAATCTATT
TTCTTATATAACTAGGAAAAAAAAGATTTCAAATAGAGCAAGTAACAAT

Last Updated on 06.12.2018 16:11