Sequences from confirmation amplicon


Confirmation sequences for line 121F08 and hit At5g10350 are shown.
Line ID 121F08
Locus At5g10350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3256981
Primers used for PCR 19ra - 8474, show primer details
   
Sequence Name 78-K115895-0022-121-F08C-19ra
GenBank Accession LN513733 [GenBank]
Sequencing primer gene specific primer, 19ra: 5'-AGAGGAGATTAAAGGAGATGGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K115895-0022-121-F08C-19ra
AGCAAAGTCGATAAGAATGGGAGCTACTCAAGGTTTTGTGACTCTTTCGTTTTTTTTTTG
AATTCGTTTGCTCGAGACTATCTTTGAATTTGATATACTAGGAGGAATCTGTTGATTGTG
TAGATTACTTTTAGGAAATTGAACTTCTGTTTCTTGCTTAACAATTTGTTTCAAATCCTG
CTAGTATGGCTGCAAATCAAGAAGGAAAGGAGGAGGTGGATGCTCGATCATTTTATGTTG
GCAATGTAAAATAGCTTATTTTCTACTTCAGGGTATCTCTCCTGTGCTTGAATCATTGTT
TCATCTAAACGGCTTTGATCTGTTTGCCAATCTTAGTGTGGATTAGCTTAGGCTGACTAT
TTTGTTAACTTAAATTTCATCTTTGATAAAAAAACTGCTTGCAATTGTGTCTAACCTTTG
TTCTTTATCCCTTGAGATTACTGTTAGAATCTGTATCAAGAGGCTTTCTCTAAATTTAAT
GTTGTGAAGTAGGTTCTTTATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAAATTTCCGCA
   
Sequence Name 48-K115894-0022-121-F08C-8409
GenBank Accession LN506604 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K115894-0022-121-F08C-8409
GACCTACTTCACAACATTAAATTTAGAGAAAGCCTCTTGATACAGATTCTAACAGTAATC
TCAAGGGATAAAGAACAAAGCTAAGACACAATTGCAAGCAGTTCTTTTATCAAAGATGAA
ATTTAAGTTAACAAAATAGTCAGCCTAAGCTAATCCACACTAAGATTGGCAAACAGATCA
AAGCCGTTTAGATGAAACAATGATTCAAGCACAGAAGAGATACCATGAAGTAGAAAATAA
GCTATCTTACATTGCCAACATAAACTGATCGAGCATCCACCTCCTCCTTTCCTTCTTGAT
TTGCAGCCATACTAGCAGGATCTGAAACAAATTGTTAAGCAAGAAACAGAAGTTCAATTT
CCTAAAAGTAATCTACACAATCAACAGATTCCTCCTAGTATATCAAATTCAAAGATAGTC
TCGAGCAAACGAATTCAAAAAAAAAACGAAAGAGTCACAAAACCTTGAGTAGCTCCCATT
TCTTTTTCGACTTTTGCTTGCATCTCTGGTAGCGCAGCAGCTTCTTCCTCCATCTCCTT

Last Updated on 06.12.2018 16:11