Sequences from confirmation amplicon


Confirmation sequences for line 121G05 and hit At1g50010 are shown.
Line ID 121G05
Locus At1g50010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18519376
Primers used for PCR BF23 - 3144, show primer details
   
Sequence Name 44-K028748-022-121-G05C-3144
GenBank Accession LN505063 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K028748-022-121-G05C-3144
GGGGCATAGGAGGAAAGCATCGAAGTGGATTCTTGGGTATGGGACCAAGTTGGTTTGGAA
CTCAGTCACATCAACATTCAAGGCACCATCGAACCTCAGAGAAGCAGTCA
   
Sequence Name 44-K028748-022-121-G05C-BF23
GenBank Accession LN505064 [GenBank]
Sequencing primer gene specific primer, BF23: 5'-GGTTATCTCATCCTTGACTGCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K028748-022-121-G05C-BF23
AACCAACTTGGNTCCCATACCCAAGAATCCACTTCATGCTTTCCTCCTATGCCCCAGTCA
TCTCCGCAGAGAAGGCCTTCCATGAGCAGCTCTCGGTTGCTGAGATCACAAACAGTGCTT
TTGAGCCAGCATCCATGATGGCAAAGTGTGACCCTCGTCACGGAAAGTACATGGCTTGCT
GTTTGATGTACCGTGGTGATGTCGTCCAATGAGTATCTGATCCCCGGGTACCCTGTCCTC
TCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGT
GCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTT
GGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTTG
Primers used for PCR BF22 - 8474, show primer details
   
Sequence Name 54-K028749-022-121-G05C-8409
GenBank Accession LN508364 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K028749-022-121-G05C-8409
GTACTAAGAAGGTTGTCGGTTTTATGTGGGATTTCTATTATCTCGTGTTTGTGTGAATAG
GCTCGAAACTCTTACGAGTCGTACTTGTGTTTTCAAAAACCATATTTCTATCTCTTGCTT
GTGGCGGTAGCCATTTGTTCTAGTTCGTTTGTTTCTGATAATATTTTCATCTCCAAGAAC
TGAATTTAAGCTGGGTTTGGTTTAAATCGATTGTCCAAAATCTAAACAAATAGAAACGTC
AAATTGTTATTTGAGTGGTGAAAGA
   
Sequence Name 54-K028749-022-121-G05C-BF22
GenBank Accession LN508365 [GenBank]
Sequencing primer gene specific primer, BF22: 5'-TTCCAAAAATTTCAATCTTTCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K028749-022-121-G05C-BF22
TAGATTTTGGACAATCGATTTAAACCAAACCCAGCTTAAATTCAGTTCTTGGAGATGAAA
ATATTATCAGAAACAAACGAACTAGAACAAATGGCTACCGCCACAAGCAAGAGATAGAAA
TATGGTTTTTGAAAACACAAGTACGACTCGTAAGAGTTTCGAGCCTATTCACACAAACAC
GAGATAATAGAAATCCCACATAAAACCGACAACCTTCTTAGTACTCCTCTCCTTCATCAT
CCTCATCGTCACCACCTTCAGCACCAACCTCTTCGTAATCCTTCTCCAATGCTGCAAGAT
CCTCACGAGCCTCTGAAAATTCTCCTTCTTCCATACCCTCACCCACATACCAGTGAACGA
AAGCACGTTTTGCGTACATAAGATCAAACTTGTGATCAATACGGGAGAACACCTCAGCAA
CACTGGTGGAGTTTGAGATCATACACACAGCTCTCTGGACTTTAGCAAGATCACCTCCTG
GAACAACAGTTGGTGGCTGGTAGTTGATTCCACACTTGAATCCAGTAGGACACCAGTCCA
CAAACTGAATAGTACGCTTGGTCTTGATGGTCCATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11