Sequences from confirmation amplicon


Confirmation sequences for line 122H04 and hit At5g37475 are shown.
Line ID 122H04
Locus At5g37475 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14866303
Primers used for PCR 10uv - 8474, show primer details
   
Sequence Name 30-K117403-0022-122-H04C-10uv
GenBank Accession LN499893 [GenBank]
Sequencing primer gene specific primer, 10uv: 5'-ATCACTTTACGCCAAAGACTTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117403-0022-122-H04C-10uv
TGATTCTCACTATTGATTACTCTTCACACTACATGTTTAGGTCTTCTTCGAGTGTATATC
TCTTGTAATGTGTTAGATTTGCTGGTTCATTATCGAAAACTTATCTTACTAATGTGGCTT
CAAATTCTAAGTGAATCATAGGCCGTGCTCTTAGTACTCGTTCAATTCATATAATAGGAA
ATAGTGTGCCCACAACAAATCTTATTACACAAATTTTTAAATCAAACCGAGCAAAGAAAA
CAGAAGAAAAACACTTTTGCAATTCAGTATACAAAACAAAAAACCGCCGACGATTTAAAC
CTCTTCTCAGTTACAATCCTATAGGAGGTAAAATGCAATCTCCAAAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 06-K117402-0022-122-H04C-8409
GenBank Accession LN488989 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K117402-0022-122-H04C-8409
ATTTTACCTCCTATAGGATTGTAACTGAGAAGAAGGTTTAAATCGTCGGCGGTTTTTTGT
TTTGTATACTGAATTGCAAAAGTGTTTTTCTTCTGTTTTCTTTGCTCGGTTTGATTTAAA
AATTTGTGTAATAAGATTTGTTGTGGGCACACTATTTCCTATTATATGAATTGAACGAGT
ACTAAGAGCACGGCCTATGATTCACTTAGAATTTGAAGCCACATTAGTAAGATAAGTTTT
CGATAATGAACCAGCAAATCTAACACATTACAAGAGATATACACTCGAAGAAGACCTAAA
CATGTAGTGTGAAGAGTAATCAATACGTAGAGAATCAAATGAACTGGAGAATCTTCGGGG
AGTAGCTTGATCAAGTCTTTGCTTAAAAAGTGAT

Last Updated on 06.12.2018 16:11