Sequences from confirmation amplicon


Confirmation sequences for line 122H06 and hit At5g37470 are shown.
Line ID 122H06
Locus At5g37470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14863183
Primers used for PCR 01xv - 8474, show primer details
   
Sequence Name 40-K119480-0022-122-H06C-01xv
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 01xv: 5'-GTCATCACAAAATGACGTTGTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K119480-0022-122-H06C-01xv
CACACGAACTTCTCGAGATGTTTAAGCCCTTGTACCAAAAACTGTTCCATTCCCTTTTGC
ACTAGCATCTTCACTGAGTTCACAAGAATGTTCAGAAGAATCCTTATCAAATCAACTCTC
ATCTCTGTATCCAAAGTCTGAATCCCCATTTTCACAACAGTTTCTAAAGCCAAGCTCCAA
TCGTCAACTCTATCTTTCTCAGGATTAAACAAAACCTTCTCAGCTTTCTCAACAATTACC
TTCATAAACTTGTCGATAAAACTCTGATACAATGATGGCAAATCGATAAAGACATGAAAA
GCCTTGACAGGTTCGTTTTCCGCTAGCCAAAGGATACAATCACTGAGCTCGTACCATCCA
CCATTATCGCAAATCACAACATTGTAGGTTATTAAAGAAACGATTCTTCCTAGGAACTTC
ATGTCAGATTCTTTGGTTTCTTGCATCGTTAAACATTCAATCACAAGGGGTTTGATTTCA
TGGAGAGCGACGCGAGAGAGTTCCCAGTTACGATTTCTGAAAACGACGAGGGTTTCGGAG
AGGAGATAAAGCGAATAGAATCTGATGACGCCATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 16-K119479-0022-122-H06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K119479-0022-122-H06C-8409
TGGCGTCATCAGATTCTATTCGCTTTATCTCCTCTCCGAAACCCTCGTCGTTTTCAGAAA
TCGTAACTGGGAACTCTCTCGCGTCGCTCTCCATGAAATCAAACCCCTTGTGATTGAATG
TTTAACGATGCAAGAAACCAAAGAATCTGACATGAAGTTCCTAGGAAGAATCGTTTCTTT
AATAACCTACAATGTTGTGATTTGCGATAATGGTGGATGGTACGAGCTCAGTGATTGTAT
CCTTTGGCTAGCGGAAAACGAACCTGTCAAGGCTTTTCATGTCTTTATCGATTTGCCATC
ATTGTATCAGAGTTTTATCGACAAGTTTATGAAGGTAATTGTTGAGAAAGCTGAGAAGGT
TTTGTTTAATCCTGAGAAAGATAGAGTTGACGATTGGAGCTTGGCTTTAGAAACTGTTGT
GAAAATGGGGATTCAGACTTTGGATACAGAGATGAGAGTTGATTTGATAAGGATTCTTCT
GAACATTCTTGTGAACTCAGTGAAGATGCTAGTGCAAAAGGGAATGGAACAGTTTTTGGT
ACAAGGGCTTAAACATCTCGAGAAGTTCGTGTTGCGAGATAAGAATCTATATAATTACAC
TAAGGTACAACGTCATTTGGTGATGAC

Last Updated on 06.12.2018 16:11