Sequences from confirmation amplicon


Confirmation sequences for line 123D09 and hit At1g75960 are shown.
Line ID 123D09
Locus At1g75960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28518804
Primers used for PCR MS34 - 8474, show primer details
   
Sequence Name 22-K016594-022-123-D09C-8409
GenBank Accession LN495895 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K016594-022-123-D09C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCAGGGGTATATTCGTC
ATGACGCTTGATTCCCTAACCGATTGGGCCGTACCGAAAACCCCGGTTTACTTATGGACC
TTACCGATATTTCACGCCAACGGTTGGACCTATCCATGGGGAATCGCCGCCGTCGGAGGA
ACTAACGTCTGTGTGCGTAAACTCCACGCGCCGTCAATATACCATCTAATCCGTGATCAC
GGCGTGACTCACATGTACGGCGCACCAATAGTGCTTCAGATTCTATCGGCGAGTCAAGAA
TCTGATCAGCCTCTTAAGAGTCCGGTCAATTTCTTAACCGCCGGTTCTTCTCCGCCAGCT
ACGGTGCTTCTCCGCGCCGAGTCTCTAGGTTTCATCGTCAGTCACGGTTACGGATTAACG
GAGACAGCCGGTGTGATCGTCTCCTGCGCGTGGAAGCCAAACTGGAATCGGTTACCGGCG
AGTGATCAAGCGCAATTGAAATCACGGCAAGGAGTGAGAACCGTCGGATTTAGCGAAATC
GATGTAGTGGATCCAGAATCAGGTCGGAGCGTGGAGAGAGACGGAGAAACAGT
   
Sequence Name 26-K016594-022-123-D09C-MS34
GenBank Accession LN497696 [GenBank]
Sequencing primer gene specific primer, MS34: 5'-CACTATTTCTCCGACTGTTTCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K016594-022-123-D09C-ms34
CTCTCCCGCTCCGACCTGATTCTGGATCCACTACATCGATTTCGCTAAATCCGACGGTTC
TCACTCCTTGCCGTGATTTCAATTGCGCTTGATCACTCGCCGGTAACCGATTCCAGTTTG
GCTTCCACGCGCAGGAGACGATCACACCGGCTGTCTCCGTTAATCCGTAACCGTGACTGA
CGATGAAACCTAGAGACTCGGCGCGGAGAAGCACCGTAGCTGGCGGAGAAGAACCGGCGG
TTAAGAAATTGACCGGACTCTTAAGAGGCTGATCAGATTCTTGACTCGCCGATAGAATCT
GAAGCACTATTGGTGCGCCGTACATGTGAGTCACGCCGTGATCACGGATTAGATGGTATA
TTGACGGCGCGTGGAGTTTACGCACACAGACGTTAGTTCCTCCGACGGCGGCGATTCCCC
ATGGATAGGTCCAACCGTTGGCGTGAAATATCGGTAAGGTCCATAAGTAAACCGGGGTTT
TCGGTACGGCCCAATCGGTTAGGGAATCAAGCGTCATGACGAATATACCCCTGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT

Last Updated on 06.12.2018 16:11