Sequences from confirmation amplicon


Confirmation sequences for line 123F06 and hit At2g30970 are shown.
Line ID 123F06
Locus At2g30970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13181140
Primers used for PCR PF81 - 8474, show primer details
   
Sequence Name 23-K102867-0022-123-F06C-8409
GenBank Accession LN496510 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K102867-0022-123-F06C-8409
AAGAGTGAACATGGGATATAAGGTAATTGAATGAAAGAATTAGCATTTTTTCATAAATGT
TACCTTAACTTCTTTTAGCCACAGACTCTTTAACTCTGGGTCTTCAAGAATGGTTGAGAC
CAGCTGAGCACCATGCAAAGGTGGGTTGCTGTACATTGGTCTAGCTAGCTGCTGCAATTG
ACTTTTCACAGCCACGGCTTGCTTCGGATCTTCACAAAGCACACTGCAGTAGAAGACAAA
GTTGTCATGTTCATATTCAGTTTTTACTTCATCACTAAATGAACCTGTATGATGGCTATT
TCATACAATTTGTTGACGCCTACAATCCAAATTTTTAAA
   
Sequence Name 23-K102868-0022-123-F06C-PF81
GenBank Accession LN496511 [GenBank]
Sequencing primer gene specific primer, PF81: 5'-TTTTAAAAATTTGGATTGTAGGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K102868-0022-123-F06C-PF81
CATACAGGTTCATTTAGTGATGAAGTAAAAACTGAATATGAACATGACAACTTTGTCTTC
TACTGCAGTGTGCTTTGTGAAGATCCGAAGCAAGCCGTGGCTGTGAAAAGTCAATTGCAG
CAGCTAGCTAGACCAATGTACAGCAACCCACCTTTGCATGGTGCTCAGCTGGTCTCAACC
ATTCTTGAAGACCCAGAGTTAAAGAGTCTGTGGCTAAAAGAAGTTAAGGTAACATTTATG
AAAAAATGCTAATTCTTTCATTCAATTACCTTATATCCCATGTTCACTCTTTTATATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
AAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAA
GCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCC
AAATGG

Last Updated on 06.12.2018 16:11