Sequences from confirmation amplicon


Confirmation sequences for line 124A02 and hit At2g44210 are shown.
Line ID 124A02
Locus At2g44210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18282379
Primers used for PCR KC47 - 8474, show primer details
   
Sequence Name 75-K035536-022-124-A02C-KC47
GenBank Accession LN513187 [GenBank]
Sequencing primer gene specific primer, KC47: 5'-TATGTCGAAGATGGGGTTTTCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K035536-022-124-A02C-KC47
AGCCGGATGTTGAAATGCCTAATGAGTTTAGCTTAGCTCAGATTTGGGTTTTAGGTGGAA
ATTTCAACTCTGATCTTAATAGTATTGAAGCTGGCTGGCAGGTATTTTTCTTCTTTCGGT
TTTTGATAGTTCTTTCCTGAAAAAGAAACTGTTTTTGATTCAAAGATTTGTGTTTTCAGG
TCAGCCCACAATTGTATGGTGATAATAGGACCAGACTCTTCACTTATTGGACTGTAAGTT
TCAGACTATTCAATCTAGATTTTTGTTTTTGTGTGTTTTGGCTTTCTTGATGAATCATTC
TTTTGTCTCTCACTTGTGAAAACAGAGTGATGCATACCAAGGCACAGGCTGCTATAATCT
TCTATGCTCAGGATTTGTTCAAATCAATAGAGAAATCGCAATGGGTGGATCAATCTCTCC
TTTATCAAACTATGGAAACTCTCAATATGACATCACCATACTCATCTGGAAGGTAAACCA
ACAACAAATATCCATGTTGTTACCATCTATTTAGTGGTTTAGCTATGGTTTGAGAAATTT
CAGTTTTGTTTTTTTGTTTTTTCTCAGGATCCAAAAGAAGGACATTGGTGGTTACAGTTT
GGAGAGAAATACATAATAGGATACTGGCCAGCTTCACTCTTCTCTTACTTATCAGAAAGT
GCATCGATGATCGAATGGGGAGGCGAAGTGGTTAACTCGCAGTCCGAGGAAGGACAACAC
ACAACCACTCAGATGGAAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAACAATGAGTATGATGGTCAATATGGAGAAAAAAGAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGT
   
Sequence Name 27-K035536-022-124-A02C-8409
GenBank Accession LN498289 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K035536-022-124-A02C-8409
CATTTAAATTGAATATATTTCCATCTGAGTGGTTGTGTGTTGTCCTTCCTCGGACTGCGA
GTTAACCACTTCGCCTCCCCATTCGATCATCGATGCACTTTCTGATAAGTAAGAGAAGAG
TGAAGCTGGCCAGTATCCTATTATGTATTTCTCTCCAAACTGTAACCACCAATGTCCTTC
TTTTGGATCCTGAGAAAAAACAAAAAAACAAAACTGAAATTTCTCAAACCATAGCTAAAC
CACTAAATAGATGGTAACAACATGGATATTTGTTGTTGGTTTACCTTCCAGATGAGTATG
GTGATGTCATATTGAGAGTTTCCATAGTTTGATAAAGGAGAGATTGATCCACCCATTGCG
ATTTCTCTATTGATTTGAACAAATCCTGAGCATAGAAGATTATAGCAGCCTGTGCCTTGG
TATGCATCACTCTGTTTTCACAAGTGAGAGACAAAAGAATGATTCATCAAGAAAGCCAAA
ACACACAAAAACAAAAATCTAGATTGAATAGTCTGAAACTTACAGTCCAATAAGTGAAGA
GTCTGGTCCTATTATCACCATACAATTGTGGGCTGACCTGAAAACACAAATCTTTGAATC
AAAAACAGTTTCTTTTTCAGGAAAGAACTATCAAAAACCGAAAGAAGAAAAATACCTGCC
AGCCAGCTTCAATACTATTAAGATCAGAGTTGAAATTTCCACCTAAAACCCAAATCTGAG
CTAAGCTAAACTCATTAAGCATTTCAACATCCGGCTTCCATACATTTATCTTCGCTTTAG
CCCCGTAGAAACC

Last Updated on 06.12.2018 16:11